A case of multicentric gliomas in both supra- and infratentorial regions with different histology: a case report
World Journal of Surgical Oncology volume 14, Article number: 152 (2016)
Multicentric gliomas are well-separated tumors in different locations of the brain, without anatomical continuity between lesions. We report a rare case of multicentric gliomas that occurred in both supra- and infratentorial regions with different histopathology.
A 27-year-old man was admitted to our hospital with mild motor weakness of the right leg. Magnetic resonance imaging (MRI) showed a large tumor occupying the left insula, extending to the left basal ganglia, so tumor resection was performed. Histological diagnosis was diffuse astrocytoma. Tumor cells showed sporadic immunoreactivity for p53 and negative immunostaining for epidermal growth factor receptor (EGFR). Postoperative course was uneventful, and adjuvant therapy was not performed. At 7 months after surgery, MRI disclosed a left cerebellar tumor displaying an irregular ring formation on enhancement with gadolinium (Gd) and marked peritumoral edema. MRI studies including T2-weighted imaging demonstrated that this paravermian tumor had no contact with the initial left insular tumor. In addition, MRI studies of the whole neuraxis, cytological examination of the cerebrospinal fluid, and neurological findings demonstrated that no dissemination had occurred through the subarachnoid space or as intracerebral metastases. Therefore, the second surgery was performed. Histological diagnosis was glioblastoma. Immunohistochemistry revealed that most tumor cells were positively stained for both p53 and EGFR but negatively stained for isocitrate dehydrogenase 1 (IDH1).
We reported a case of multicentric gliomas occurring in both supra- and infratentorial regions with different histopathology. Immunohistochemical examinations suggest that different genetic pathways may participate in the occurrence of these tumors.
Multicentric gliomas are well-recognized entities, but their occurrence is still rare. Among these, gliomas arising from both supra- and infratentorial regions with different histology are very rare [1–4]. We report the case of a patient with multicentric gliomas whose tumors occurred in both supra- and infratentorial regions with different histological types. One was diffuse astrocytoma in the left insula, and the other was glioblastoma in the cerebellum. We present the case and discuss the possible pathogeneses of multicentric gliomas.
A 27-year-old man began to experience disorientation and visited his family doctor. Although magnetic resonance imaging (MRI) showed an abnormal mass in the left insula, extending to the left basal ganglia, he was followed without therapy. The tumor gradually increased in size, and he was referred to our department. Neurological examination on admission revealed clear consciousness and no abnormality in cranial nerve functions other than mild weakness of the right leg (manual muscle test: 4/V). MRI on initial admission showed a tumor mass (diameter, 70 mm) extending from the left insula to the basal ganglia. The lesion was hypointense on T1-weighted imaging and hyperintense on T2-weighted imaging. The lesion was not enhanced on administration of gadolinium (Gd) (Fig. 1a–c). No other lesions were identified in the brain, including the cerebellum (Fig. 1d, e). Cerebral angiography disclosed no apparent tumor staining or abnormal vessels. As intraoperative histological examination revealed that the left insular tumor was a low-grade glioma, the tumor was partially resected under the electrophysiological monitoring using a motor-evoked potential to avoid serious neurological complications. Postoperative histological diagnosis was diffuse astrocytoma (World Health Organization (WHO) grade II astrocytoma) (Fig. 2a). Immunohistochemical studies showed that Ki-67 (MIB-1) proliferation-related labeling index was very low at 1 % or less, and tumor cells revealed sporadic immunoreactivity for p53, but a negative staining for epidermal growth factor receptor (EGFR) (Fig. 2b–d). Postoperative course was uneventful, and adjuvant therapy was not performed because of a very low MIB-1 labeling index in the tumor.
MRI performed 3 months after surgery did not show any growth of the left insular tumor or any other lesion (Fig. 3). At 7 months after surgery, the patient presented with headache, nausea, and cerebellar symptoms. MRI disclosed a left cerebellar tumor displaying an irregular ring formation on enhancement with Gd and marked peritumoral edema (Fig. 4a–c). The residual supratentorial tumor remained unchanged compared to MRI 7 months earlier (Fig. 4d, e). Total resection of the cerebellar tumor was performed (Fig. 5) and a ventriculo-peritoneal shunt was placed because of persisting hydrocephalus. Histological examination revealed that the tumor showed high cellularity, cellular and nuclear anaplasia, and mitotic figures and also had prominent microvascular proliferation and large necrosis often accompanied with pseudopalisading of tumor cells, leading to diagnose as glioblastoma (WHO grade IV astrocytoma) (Fig. 6a, b). Ki-67 proliferation-related labeling index was very high, at 42.8 %, and most tumor cells showed positive immunostains for both p53 and EGFR (Fig. 6c–e), but a negative staining for isocitrate dehydrogenase 1 (IDH1) (Fig. 6f). Extended focal radiotherapy at a total dose of 50 Gy and one course of chemotherapy comprising carboplatin and etoposide were performed. Nine months after radio-chemotherapy, the patient died of dissemination from the infratentorial tumor through the ventricle space, but residual insular tumor remained unchanged. As permission of autopsy could not be obtained from his family, we could not confirm the histology of these lesions.
The clinical study of the abovementioned case report was approved by the Ethics Committee for Clinical Research of Ehime University Hospital, and informed consent was obtained from the patient prior to initiating the study.
As for the definition of multicentric gliomas, Batzdorf and Malamud described the criteria that multifocal tumors should be distinguished from multicentric tumors . The multifocal tumors result from invasion or growth by established routes such as commissural or tract fibers. Otherwise, such tumors occur by spread via cerebrospinal fluid channels or local metastasis. In contrast, multicentric gliomas occur in a distant site to each other, such as in different lobes or hemispheres, and there is no connecting pathway between these tumors. It is also emphasized that these two tumors occur at different times. Conversely, in a brain autopsy study, Mishra et al. stated that even when tumor connectivity is missing based on macroscopic findings, the possibility of microscopic connectivity cannot be completely excluded . Malcolm et al. suggested that gliomas occurring in both supra- and infratentorial regions may be considered true multicentric gliomas, as they arise the different compartment of the brain without gross or microscopic connections . In the present case, the two tumors arose in different locations of supra- and infratentorial regions (one in the left insula; the other in the cerebellum), and no macroscopic connectivity was apparent on MRI. Although the possibility of local intracerebral metastasis could not be excluded, these two tumors were diagnosed as multicentric gliomas because they showed different histological findings.
Multicentric gliomas more often affect middle-aged men and show rapid tumor progression, and the prognosis is extremely poor. Many reported cases of multicentric gliomas occur in the supratentorial compartment, and occurrence in both supra- and infratentorial regions is very rare [4–7]. To the best of our knowledge, only 11 cases of multicentric gliomas arising above and below the tentorium have been reported, and all exhibited different histological findings [4–12]. Mean patient age is 37.8 years (range, 11–63 years). No clear gender differences have been identified. These tumors arise predominantly in the cerebral hemisphere and cerebellum, and all cerebellar tumors have been located around the fourth ventricle, as in the present case. Histological examination of these 11 patients revealed that both supra- and infratentorial tumors showed high-grade gliomas in five patients. In another five patients, when one tumor located in either supra- or infratentorial region showed high grade, the other located in the different region was low grade (including the present case). In one patient, both tumors displayed low-grade glioma. In the present case, the supratentorial tumor remained low grade, whereas the infratentorial tumor rapidly proliferated within several months. Of the past 11 reports, only one patient has been reported with infratentorial tumor rapidly progressing to a malignant type within a few months after resection of the supratentorial tumor.
The pathogenesis of multicentric gliomas remains unclear. Many hypotheses have been proposed to explain the multicentricity. Willis et al. suggested that multicentric lesions could result from a two-step process . At first, a large area of the brain, or even the entire brain, undergoes neoplastic transformation (initiation), becoming more susceptible to neoplastic growth. In the second stage (promotion), neoplastic proliferation in multiple sites occurs after various stimulations. Genetic and molecular analyses are very useful and important for elucidating the pathogenesis of multicentric gliomas.
Reis et al. investigated genetic changes to compare primary and secondary glioblastomas and reported that these tumors were independent of each other, with showing a different pattern of genetic mutations . Furthermore, the phenotype of these tumors included genetic mutations of tumor-suppressor genes such as p53, phosphatase, and tensin homologue deleted from chromosome 10 (PTEN) and overexpression of EGFR. Watanabe et al. indicated that overexpression of EGFR and mutations of p53 are mutually exclusive, and the alteration of either gene leads to different pathways for the evolution of glioblastoma as the common phenotypic endpoint . Primary glioblastoma thus occurs by a de novo pathway with overexpression of EGFR, whereas secondary glioblastoma develops by a progression pathway in which p53 mutations operate as an initiation step for neoplastic transformations less frequently with EGFR overexpression. In the present case, supratentorial tumor revealed immunoreactivity for p53, whereas EGFR showed a negative reaction. The malignant phenotype derived from this tumor is thus postulated to represent a secondary glioblastoma. However, facts that the infratentorial tumor showed positive immunoreactivity for both p53 and EGFR, displayed wild-type IDH1, and had large ischemic necrosis might make a contradiction if the tumor developed along the progression pathway [15, 16]. In the present case, p53 mutations may have occurred throughout the brain, possibly inducing transformation of the glial cells as described by Willis et al. The supratentorial tumor may have developed from a genetic background of p53 mutations. Taking into consideration the fact that the infratentorial tumor occurred within a short period of less than 4 months, the malignant phenotype may have developed through a de novo pathway. These may be the reasons for positive stainings of both p53 and EGFR in the infratentoral tumor. To elucidate the pathogenesis of multicentric gliomas, further genetic analysis of glioma oncogenesis-related genes including these molecules will be necessary with obtaining more critical information.
We reported a case of multicentric gliomas occurring in the supra- and infratentorial regions with different histopathology. One tumor was diffuse astrocytoma of the left insula, while the other was glioblastoma of the cerebellum. Immunohistochemical analysis suggested that these tumors might have occurred by different pathways of neoplastic transformation in glial cells.
EGFR, epidermal growth factor receptor; Gd, gadolinium; IDH1, isocitrate dehydrogenase 1; MRI, magnetic resonance imaging; PTEN, phosphatase and tensin homologue deleted from chromosome 10.
Hassaneen W, Levine NB, Suki D, Salaskar AL, de Moura LA, McCutcheon IE, et al. Multiple craniotomies in the management of multifocal and multicentric glioblastoma. Clinical article. J Neurosurg. 2011;114:576–84.
Kaku S, Terao T, Taya K, Ohtuka T, Tanaka T, Sawauchi S, et al. A multicentric glioma presenting different pathological appearances: a case report. No Shinkei Geka. 2004;32:501–6.
Batzdorf U, Malamud N. The problem of multicentric gliomas. J Neurosurg. 1963;20:122–36.
Mishra HB, Haran RP, Singh JP, Joseph T. Multicentric gliomas: two case reports and a review of the literature. Br J Neurosurg. 1990;4:535–9.
Malcolm FP, Thomas R, David GT. Multicentric malignant glioma. Br J Neurosurg. 1991;5:631–4.
Hiroyuki N, Manabu H, Hideaki I. An autopsy case of multicentric glioma of multiple histopathology. No Shinkei Geka. 1987;15:1073–7.
Kotwica Z, Papierz W. Cerebral and cerebellar glial tumors in the same individual. Neurosurgery. 1992;30:439–41.
Bradley WL. Case of gliosarcomatose tumours of the cerebrum and cerebellum. Pro Conn Med Soc. 1880;2:39–41.
Misra BK, Steers AJ, Miller JD, Gordon A. Multicentric glioma presenting with hemorrhage. Surg Neurol. 1988;29:73–6.
Salvati M, Caroli E, Orlando ER, Frati A, Artizzu S, Ferrante L. Multicentric glioma: our experience in 25 patients and critical review of the literature. Neurosurg Rev. 2003;26:275–9.
Solitare GB. Cerebellar and cerebral gliomas occurring in the same individual. J Neurosurg. 1962;19:1079–84.
Solomon A, Perret GE, McCormick WF. Multicentric gliomas of the cerebral and cerebellar hemispheres: case report. J Neurosurg. 1969;31:87–93.
Willis RA. Pathology of tumors. London: Butterworth; 1960. p. 811.
Reis RM, Herva R, Brandner S, Koivukangas J, Mironov N, Bär W, et al. Second primary glioblastoma. J Neuropathol Exp Neurol. 2001;60:208–15.
Watanabe K, Tachibana O, Sato K, Yonekawa Y, Kleihues P, Ohgaki H. Overexpression of the EGF receptor and p53 mutations are mutually exclusive in the evolution of primary and secondary glioblastomas. Brain Pathol. 1996;6:217–24.
Homma T, Fukushima T, Vaccarella S, Yonekawa Y, Di Patre PL, Franceschi S, Ohgaki H. Correlation among pathology, genotype, and patient outcomes in glioblastoma. J Neuropathol Exp Neurol. 2006;65:846–54.
The authors would like to express their gratitude to Taichi Furumochi and Yasuhiro Shiraishi, Department of Neurological center, Ehime University Hospital, Japan, for their helpful information regarding radiological image acquisition.
This research received no specific grant from any funding agency in the public, commercial, or not-for-profit sectors. Furthermore, none of the authors have any commercial or financial involvement in connection with this study that represent or appear to represent any conflicts of interest.
Availability of data and materials
Informed consent was obtained from the patient prior to initiating the study. The datasets supporting the conclusions of this article are available in the repository of Ethics Committee for Clinical Research of Ehime University Hospital.
AI wrote the manuscript. AI, TO, SK, and SO performed the surgery. TO, YM, RK, and YN drafted the manuscript. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
Consent for publication
Written informed consent was obtained from the patients for publication of this case report and any accompanying images. A copy of the written consent is available for review by the Editor-in-Chief of this journal.
Ethics approval and consent to participate
The clinical study of the abovementioned case report was approved by the Ethics Committee for Clinical Research of Ehime University Hospital.
About this article
Cite this article
Inoue, A., Ohnishi, T., Kohno, S. et al. A case of multicentric gliomas in both supra- and infratentorial regions with different histology: a case report. World J Surg Onc 14, 152 (2016). https://doi.org/10.1186/s12957-016-0907-4