Fig. 6

Photomicrographs showing histopathology of the cerebellar tumor. Microscopic histology (a) displayed the tumor with high cellularity, increased tumor vessels, and a pseudopalisading of tumor cells around large necrosis. The microscopic view with high magnification (b) revealed tumor cells with marked cellular and nuclear anaplasia and the prominent proliferation of vascular endothelial cells and necrosis (hematoxylin and eosin, a ×100, scale bar: 400 μm, b ×400, scale bar: 50 μm). Immunohistochemistry for MIB-1 showing a high Ki-67 (MIB-1) labeling index (42.8 %) (c). Immunohistochemistry for p53 (d) and EGFR (e) disclosing strongly positive immunoreactivity of tumor cells for both molecules, but a negative staining for IDH1 (f). c–f ×400, scale bar: 50 μm