- Open Access
Perivascular epithelioid cell tumor (PEComa) of the uterine cervix associated with intraabdominal "PEComatosis": A clinicopathological study with comparative genomic hybridization analysis
© Fadare et al; licensee BioMed Central Ltd. 2004
- Received: 10 September 2004
- Accepted: 19 October 2004
- Published: 19 October 2004
The Erratum to this article has been published in World Journal of Surgical Oncology 2005 3:25
The World Health Organization recently recognized a family of neoplasms showing at least partial morphological or immunohistochemical evidence of a putative perivascular epithelioid cell (PEC) differentiation. These tumors include angiomyolipoma (AML), clear cell "sugar" tumors of the lung (CCST), lymphangioleiomyomatosis (LAM), clear cell myomelanocytic tumors of the falciform ligament and distinctive clear cell tumors at various other anatomic sites.
Case presentation & methods
A 41-year old gravida-1 para-1 with tuberous sclerosis presented with an incidentally identified 2.2 cm mass. The morphology and immunohistochemical profile was consistent with PEComa. Distinct aggregates of HMB-45 epithelioid cells were present in an occasionally distinctive perivascular distribution in the myometrium, small bowel lamina propria and ovarian hila. These distinctive aggregates, for which we propose the designation "PEComatosis" based on their intraabdominal distribution, did not display cytological atypia, mitotic activity or necrosis. CGH and DNA ploidy analysis showed a balanced chromosomal profile and diploid nuclei, respectively. There was no recurrence or metastases at 35 months' follow-up. Fifty-one previously reported cases of non-AML, LAM and CCST PEComas [perivascular epithelioid cell tumors- not otherwise specified (PEComa-NOS)] are reviewed.
The lesions may be a reflection of tumor multicentricity, in which each may be a potential nidus for the development of future more well-developed tumors. Alternatively, they may be a manifestation of a poorly understood "field effect", in which there is an increased propensity to develop tumors of this type throughout the abdomen. Finally, and least likely in our opinion, they may represent tumor spread from its primary site.
- Tuberous Sclerosis
- Tuberous Sclerosis Complex
- Uterine Cervix
- Adnexal Mass
- Smooth Muscle Tumor
Perivascular epithelioid cell tumors (PEComa) have been the subject of abundant discussion in the medical literature over the past decade [1–47]. Morphologic and immunophenotypic similarities between some constituent cells of renal angiomyolipomas (AML) and those of a case of clear cell "sugar" tumor of the lung (CCST) were initially noted in 1991 . One year later, Bonetti et al , formally proposed the concept of "perivascular epithelioid cell" (PEC), a then provisional term meant to describe the epithelioid cells that characterize, at least in part, the aforementioned lesions. Characteristics of PEC (which does not have a normal anatomic homologue) include co-expression for melanocytic and muscle markers, epithelioid to spindle cellular shapes with ample clear to eosinophilic cytoplasm, and at least in some cases, arrangement around blood vessels . Ultrastructurally, structures interpreted as melanosomes and premelanosomes have been demonstrated in some tumors composed of PECs [14, 18, 31, 38], but not in others [12, 19, 20, 28, 41]; an additional case showed macroscopic pigmentation . In 1994, based on the morphologic and immunophenotypic distinctiveness of PECs, in addition to the fact that similar cells had been described in some other tumors, Bonetti et al proposed the concept of a family of lesions sharing this cellular phenotype, including CCST, AML, and lymphangioleiomyomatosis (LAM) . The term "PEComa" was introduced by Zamboni et al  in 1996 as synonym for this family of tumors.
Over the past decade, PEC and tumors composed of them have engendered significant discussions and controversies with respect to their very existence as a clinico-pathological entity, their histogenesis, pathogenesis, and nomenclature [2–6, 16, 17, 25, 26, 32, 33, 35, 39, 40]. Nonetheless, in 2002 and 2003, two monographs published under the auspices of the World Health Organization (WHO) recognized a family of neoplasms with perivascular epithelioid cell differentiation and accepted the designation "PEComa" [13, 21]. In the WHO soft tissue volume, PEComas are defined as "mesenchymal tumors composed of histologically and immunohistochemically distinctive perivascular epithelioid cells" . Members of the PEComa family that were recognized include AML, CCST, lymphangioleiomyomatosis (LAM), clear cell myomelanocytic tumor (CCMMT) of the falciform ligament/ ligamentum teres and a heterogeneous group of other "unusual clear cell tumors" at various anatomic sites . The latter group includes tumors that have been reported under varying designations, such as abdominopelvic sarcoma of perivascular epithelioid cells , primary extrapulmonary sugar tumor (PEST) , clear cell myomelanocytic tumors of the skin  and thigh , and simply PEComa of various anatomic sites [1, 9, 12, 19, 24, 27, 28, 31, 40, 41, 45, 46]; these, in addition to CCMMT of the falciform ligament  will henceforth be referred to as PEComa n ot o therwise s pecified (PEComa NOS). This descriptive designation, as used in this report, excludes the well-established entities LAM, CCST of the lungs and all variants of AML. Most of the reported cases of PEComa NOS have been tumors located in the uterine corpus (21/51; 41%); however, consequent to the publication of the WHO monographs, there has been a recent noticeable increase in the number of reported cases of PEComa NOS, with almost 70% of all cases reported between 2001 and 2004 [1, 6–8, 10–12, 15, 19, 20, 22, 24, 27, 28, 31, 38, 40, 41, 43–46](additional file 1). Concurrently, there has been an increase in the diversity of anatomic sites from which they reportedly arose, such that, it now appears that these tumors may potentially arise from any anatomic location. The morphologic and immunophenotypic spectrum as well as exclusion/inclusion criteria of PEComa NOS are not well-defined, and pathologic parameters with prognostic value have yet to be elucidated. In addition, there is a striking scarcity of information on the molecular and cytogenetic data of these lesions, probably due to their rarity. In this report, we present an example of a PEComa NOS of the uterine cervix, with the following objectives: 1) to document the uterine cervix as another potential site for a PEComa NOS, 2) based on the presence of tumorlets outside of their primary site, to analyze morphologic criteria predictive of clinical behavior of PEComa NOS, 3) to present CGH and ploidy analysis of PEComa NOS, and 4) to present another example of a PEComa NOS occurring in a tuberous sclerosis patient, an association which has been documented only twice previously [6, 40]).
Immunohistochemistry: antibody specifications and results
Antigen retrieval method
DakoCytomation Carpinteria, CA, USA
Ventana, Tucson AZ, USA
Progesterone receptor (PR)
Estrogen receptor (ER)
Smooth muscle actin (SMA)
Sigma, St Louis, MO, USA
Chemicon Int, Temecula, CA
Of the 51 cases of PEComa NOS that have been documented in the literature [1, 6–8, 10–12, 14, 15, 19, 20, 22–25, 27, 28, 31, 32, 34, 37, 38, 40–46], 90% (46/51) developed in females and 41% (21/51) were described in the uterine corpus. Follow-up information was unavailable (n = 7) or too recent at the time of the report (n = 4) in 22% of cases [7, 10, 14, 15, 34, 40, 43, 46]. One patient whose tumor was primary in the left atrium died postoperatively of "cardiac failure thought to be due to nontumorous coronary artery thromoboemboli" . Two cases were excluded from the following analysis (44, 45) based on insufficient morphologic information (44) or inconsistence with our analytic paradigm (45). Of the remaining 37 cases (additional file 2), the associated tumors behaved in a benign fashion in 25 cases (68%). These tumors were confined to their respective primary sites and showed no evidence of recurrence or metastases with follow-up periods that ranged from 6 weeks to 22 years, and will hereafter be referred to as the "benign cases" [1, 6, 8, 14, 20, 23, 25, 27, 32, 37, 38, 40, 42, 44]. However in twelve cases, the tumors recurred after apparently complete surgical resections, were either metastatic at presentation or metastasized after long periods. Four of these cases were ultimately fatal [6, 19, 22, 28], and all 12 will hereafter be referred to as the "non-benign cases" [6, 11, 12, 14, 19, 22, 24, 28, 31, 41].
Clinical parameters were not particularly discriminatory between the two groups: the average patient age of the benign cases (37 years) was not significantly different from that of the non-benign cases (43 years) [p = 0.56] and there was a diversity of clinical presentations related primarily to the sites of origin. The non-benign cases (mean diameter of 11 cases: 7.5 cm) tended to be larger than the benign cases (mean diameter of 24 cases: 4.54 cm); the statistical significance of the difference (p = 0.022) is maintained even when the outlier effect of the 1 case with a 20 cm diameter tumor reported by Folpe et al  in the non-benign group is removed. Morphologic features in the benign and non-benign cases are compared below.
We have presented herein the first documented case of a PEComa NOS of the uterine cervix. In addition to the primary tumor, aggregates of HMB-45+ clear cells were present at several other intraabdominal sites, including the small bowel lamina propria, ovarian hila and myometrium. Based on this pattern of distribution of tumor cells, we propose the designation "PEComatosis" to describe such aggregates. The morphogenetic basis for the intraabdominal lesions remains unclear. The similarity in morphologic features and immunophenotype between the cervical and extracervical lesions suggests that either a) both lesions arise from the same site or progenitor, or b) they both represent tissue responses of different degrees to the same stimulus. Several possibilities were considered, all of which are necessarily speculative. The lesions may be a reflection of tumor multicentricity, in which each may be a potential nidus for the development of future more well-developed tumors. Alternatively, they may be a manifestation of a poorly understood "field effect", in which there is an increased propensity to develop tumors of this type throughout the abdomen. Finally, and least likely in our opinion, they represented the tumor spread from its primary site.
The diffuse occurrence of apparently heterologous tissue is a well-known phenomenon in the peritoneal cavity. These include leiomyomatosis peritonealis disseminata (LPD), gliomatosis peritonei and the recently described diffuse cartilaginous metaplasia of the peritoneum [50, 52]. In LPD, for example, diffusely distributed peritoneal nodules of benign smooth muscle may proliferate or regress based on the hormonal milieu of pregnancy . In this instance, circulating hormone levels are thought to represent the main stimuli underlying the proliferation of the peritoneal lesions. Even though a uterine smooth muscle tumor is associated with most cases, they are not thought to be the origin of LPD . In contrast, gliomatosis peritonei is believed to represent an overgrowth of glial implants from ovarian teratomas . In either instance, the presence of diffuse intraperitoneal lesions associated with these benign lesions (an ovarian teratoma and a uterine leiomyoma) is deemed insufficient to assign them a malignancy status . These examples are directly relevant to our case, in which a malignant potential has to be assigned to a pathologically benign tumor that is showing similar appearing cells remote from its primary site. In the current case, the distinctive tropism of tumor cells for vascular structures without true intraluminal foci, argues against a hematogenous spread of tumor and argues for a de novo proliferation of PEC at those sites. However, whether these are foci that would have undergone involution or continued proliferating into tumor masses remains unclear. Do these lesions represent a manifestation of a poorly understood "field effect", in which there is an increased propensity to develop tumors of this type throughout abdomen? The most obvious underlying condition in this particular patient is tuberous sclerosis. A potentially comparable condition may exist in the lungs. In a few patients with and without tuberous sclerosis, a distinctive diffuse pulmonary interstitial proliferation has been described [53–56]. These proliferations are composed of clear HMB-45+ cells and are inconstantly associated with LAM [50–53]. Whether a similar mechanism is operational here is not clear. Although we do not believe the extracervical lesions represent metastases, it should be noted that contrary to the conventional paradigm, molecular evidence is accumulating regarding the "metastasis of benign tumor cells" in patients with tuberous sclerosis, although this is currently limited to the renal angiomyolipoma to pulmonary lymphangioleiomyomatosis model .
The issues raised by this case highlight the absence of well-established morphologic criteria predicting aggressiveness or malignancy in PEComas. Based on an analysis of 31 of the 51 reported cases detailed in Additional file 1, the following information is stated in the WHO monograph regarding the aforementioned criteria : "it appears that PEComas displaying any combination of infiltrative growth, marked hypercellularity, nuclear enlargement and hyperchromasia, high mitotic activity, atypical mitotic figures, and coagulative necrosis should be regarded as malignant". However, while the presence of all the mentioned features would probably assign malignancy to any tumor, it is unclear what significance there is of the presence or absence of individual features or small combinations thereof.
Since the relevance of any set of pathologic criteria is ultimately dependent on their correlation to clinical behavior based on published experience, we analyzed in greater detail the clinicopathologic features of those 12 cases in which aggressive behavior was already manifest and compared them to those of the 25 cases with benign outcomes. It is well recognized by the authors that this separation is artificial, the definitional threshold for 'aggressiveness" is low, and that for example the recurrence of a tumor is by no means necessarily indicative of its malignancy. Nonetheless, this separation allows comparative analysis of groups of cases whose clinical behaviors have been shown to be different.
With regard to morphologic appearance, some features appeared to distinguish the two groups. Nuclear atypia (nucleomegaly, multinucleation, pleomorphism etc) was more likely to be present in the non-benign group, with this feature described in 8 of the 10 cases in which a comment was rendered. However, some degree of nuclear atypia was also described in 9 of the 25 cases in the benign group; the atypia in all of the latter cases were described as "minimal" or mild to moderate. Two cases that, in our opinion, showed the highest degree of nuclear atypia (in addition to high mitotic activity and necrosis) were unfortunately reported without follow-up information [10, 34].
Mitotic activity was uniformly low in the benign group, with no mitotic figures identified in 13/25 (52%) cases and rare (<1/20HPF) mitotic figures found in the remainder with information. However, for the purposes of answering the more clinically relevant question, i.e. segregation of the non-benign group, mitotic activity was not useful. Only 3 of the 10 non-benign cases (in which mitotic activity information was given) showed significant mitotic activity (≥ 6/10HPF). In the remaining 7 cases, mitotic figures were described as "rare" (n = 3), "low" (n = 2) and <1/20hpF (n = 2).
Necrosis was a common feature of cases in the non-benign group, being present in 7 of 11 cases (64%), with an 8th case described as showing "gelatinous-appearing material" macroscopically . In contrast, of the benign group, necrosis was present in only 4 of the 18 (22%) cases in which such information was stated. Additionally, the necrosis in one of those 4 cases was described as "infarct-type" (non-coagulative) . However, one of the benign cases was described as showing "scattered foci of coagulative necrosis and hemorrhage" . As can be anticipated, lymphovascular invasion (LVI) by tumor was more characteristic of tumors in the non-benign group as compared to their benign counterparts: LVI was present in 4 out of 8 (50%) cases in the non-benign group and in only one case in the benign group. The latter is Case 3 of the "abdominopelvic sarcomas" described by Bonetti et al ; this tumor was a 2.5 cm well-circumscribed pelvic nodule, the patient showed no evidence of tumor recurrence or metastases at 6 months follow-up. For the purposes of this analysis, we placed this case in the benign group, definitionally based on the benign follow-up.
We also analyzed the degree and types of tumor infiltration as another potential discriminator between the benign and non-benign groups. In what remains the largest series of PEComa NOS reported to date, Vang and Kempson  divided 8 uterine PEComas into 2 groups (A and B) based on, in part, the degree of tumor infiltration. Of the group A, 75% of tumors showed a tongue-like myometrial infiltration reminiscent of low-grade endometrial stromal sarcoma while this type of infiltration was only focal in 75% of their group B tumors; all cases were limited to the uterus. Due to the absence of follow-up in 75% of their group A cases, the prognostic significance of this classification is unclear. Two of the four "hyalinized uterine mesenchymal neoplasms with HMB-45-positive epithelioid cells" reported by Michal and Zamecnik  showed a similar "tongue-like" infiltration and had a benign follow-up. All of the 7 cases of CCMMT reported by Folpe et al  "appeared circumscribed but displayed an infiltrative pattern microscopically at the periphery", a pattern remarkably similar to our case. One of their 6 cases with follow-up showed evidence of pulmonary metastases at 3 months, while follow-up was unremarkable in others. This patient reportedly died of other causes. Analysis of the usefulness of infiltration was hampered somewhat by the absence of a comment on this subject in some of the non-benign cases; however, it is unlikely to be a criterion of significant use in isolation. Only in 3 of 10 malignant cases infiltration was prominent. In 4 of the 7 remaining cases in the non-benign group, infiltration was not specifically noted; the latter includes a well-circumscribed and encapsulated 9 cm mass involving the terminal ileum and cecum  which metastasized to the liver and the patient died in 28 months. The 7th and 8th cases showed only local infiltration [11, 14]. Another case reported by Ruco et al , which we excluded from our analysis of the non-benign cases due to an absence of outcome information, consisted of a partially necrotic 5 cm mass showing high mitotic activity (11 m/10hpF) and was described as "poorly circumscribed". One of the cases described by Fukunaga , which we have placed in the benign group showed focal infiltrative growth. For the rest of the benign group, significant infiltration was not described in any case.
Summary of pathologic features in reported cases of PEComa NOS
Non-Benign cases (n = 12)
Benign cases (n = 25)
Size (average diameters)
7.5 cm (n = 11)
4.54 cm (n = 24)
Significant mitotic activity (6 mitoses per 10 high power fields or greater
An important differential consideration for PEComas arising in the uterus is epithelioid smooth muscle tumors. Vang and Kempson  expressed the opinion that PEComas and epithelioid smooth muscle tumors "exist on a morphologic spectrum" and recommended that HMB-45 staining be performed on all epithelioid tumors of the uterus. The contrary view was expressed by Silva et al  who demonstrated immunoreactivity for HMB-45 in 4 (80%) out of 5 "unequivocal uterine leiomyosarcomas" with epithelioid features. They concluded that HMB-45 immunoreactivity is insufficient to designate these tumors as PEComas and separate them from epithelioid smooth muscle tumors. Zamecnik and Michal  found immunoreactivity for HMB-45 in four distinctively hyalinized epithelioid mesenchymal tumors of the uterus, but all four cases were negative for the other three melanogenesis markers tested (Melan-A, tyrosinase and micropthalmia transcription factor). The authors concluded that their cases were closer linked to epithelioid smooth muscle tumors than PEComas. In the report by Ruco et al , twelve uterine leiomyomas, two of which were epithelioid, were negative for HMB-45. In contrast, at least focal HMB-45 positivity was demonstrated in 43 of 79 (54%) typical (non-epithelioid) smooth muscle tumors of the uterus in 2 combined series [60, 61]. These somewhat contradictory findings illustrate that at this time, the relationship between epithelioid smooth muscle tumors and PEComas (outside of their shared co-expression of muscular markers) is unclear. However, since both tumors are rare, we agree with Vang and Kempson  that HMB-45 immunostaining should be performed on all epithelioid uterine tumors, not only to better delineate the features of both epithelioid smooth muscle tumors and PEComas, but due to the possibility of an association between the latter and the tuberous sclerosis complex (TSC).
Although the association between some members of the PEComa family (AML and LAM) and the tuberous sclerosis complex is well-known, this case represents only the third case of a PEComa NOS reported to occur in a patient with stigmata of this complex. Both of the previous cases were primary in the uterus [6, 40]. Even this seemingly low rate of association (6%; 3/50) is almost certainly higher than that associated with most tumors, and may thus warrant an investigation for features of TSC in patients in whom these tumors are diagnosed.
The validity of segregating a tumor group based almost entirely on the clear appearance of constituent cells and immunoreactivity for melanogenesis markers may be proven if recurrent molecular or cytogenetic abnormalities are identified in this group. However, remarkably sparse information exists on the cytogenetic or molecular pathogenesis of PEComa NOS. Using conventional cytogenetics, Folpe et al  identified loss of X chromosome and a t(3;10) in 1 of 5 metaphases examined from a case of CCMMT. RT-PCR analysis of one perivascular epithelioid cell tumor has failed to show the EWS/ATF-1 fusion transcript from the t(12;22) characteristic of clear cell sarcoma of soft parts – another differential consideration . No other cytogenetic analyses of PEComa NOS have been reported to our knowledge. p53 does not appear to be involved in the pathogenesis of these tumors, as neither p53 mutations as determined by single-stranded conformational pleomorphism analysis, nor protein overexpression as determined by immunohistochemistry have been identified . Our case represents the first PEComa NOS that has been studied by CGH. Although this needs to be confirmed with more cases, the absence of chromosomal gain or loss detectable by this method, in additional to a diploid DNA content of our case, suggests that karyotypical changes may not be features of PEComa NOS.
In summary, we have documented herein the first case of a PEComa NOS of the uterine cervix occurring in a tuberous sclerosis patient. With the description of additional cases, more insight into their behavior and predictive morphologic parameters may be achieved.
Additional file 1: PEComa additional file 1. doc : All reported cases of PEComa NOS
Additional file 2: PEComa additional file 2. doc: Morphologic analysis of the 37 cases of PEComa NOS with adequate follow-up information, classified by outcome
Patient consent was obtained for the presentation of her records.
A preliminary version of this study was presented at the annual meeting of the American Society of Clinical Pathologists (ASCP), San Antonio, TX, Oct 7–10, 2004.
- Adachi S, Hanada M, Kobayashi Y, Tsutahara K, Fukuhara S, Mori N, Hara T, Mukai H, Shimasaku E, Kawai M, Kishikawa N, Yamaguchi S: Heavily melanotic perivascular epithelioid cell tumor of the kidney. Pathol Int. 2004, 54: 261-265. 10.1111/j.1440-1827.2004.01617.x.View ArticlePubMedGoogle Scholar
- Bonetti F, Pea M, Martignoni G, Zamboni G: The perivascular epithelioid cell and related lesions. Adv Anat Pathol. 1997, 4: 343-358.View ArticleGoogle Scholar
- Nuciforo PG, O'Hara CD, Bonetti F, Martignoni G, Colato C, Manfrin E, Gambacorta M, Faleri M, Bacchi C, Sin VC, Wong NL, Coady M, Chan JK: Abdominopelvic sarcoma of perivascular epithelioid cells. Report of four cases in young women, one with tuberous sclerosis. Mod Pathol. 2001, 14: 563-8. 10.1038/modpathol.3880351. And Tazelaar HD, Batts KP, Srigley JR: Primary extrapulmonary sugar tumor (PEST): a report of four cases. Mod Pathol 2001, 14:615–22. Mod Pathol 2002, 15:87–90[MP1].View ArticleGoogle Scholar
- Bonetti F, Pea M, Martignoni G, Zamboni G: PEC and sugar. Am J Surg Pathol. 1992, 16: 307-308.View ArticlePubMedGoogle Scholar
- Bonetti F, Pea M, Martignoni G, Doglioni C, Zamboni G, Capelli P, Rimondi P, Andrion A: Clear cell ("sugar") tumor of the lung is strictly related to angiomyolipoma: the concept of a family of lesions characterized by the presence of the perivascular epithelioid cell (PEC). Pathology. 1994, 26: 230-236.View ArticlePubMedGoogle Scholar
- Bonetti F, Martignoni G, Colato C, Manfrin E, Gambacorta M, Faleri M, Bacchi C, Sin VC, Wong NL, Coady M, Chan JK: Abdominopelvic sarcoma of perivascular epithelioid cells: report of four cases in young women, one with tuberous sclerosis. Mod Pathol. 2001, 14: 563-568. 10.1038/modpathol.3880351.View ArticlePubMedGoogle Scholar
- Crowson AN, Taylor JR, Magro CM: Cutaneous clear cell myomelanocytic tumor-perivascular epithelioid cell tumor: first reported case [Abstract]. Mod Pathol. 2003, 16: 90A-Google Scholar
- Fukunaga M: Perivascular epithelioid cell tumor of the uterus: a case report. Int J Gynecol Pathol. 2004, 23: 287-291. 10.1097/01.pgp.0000130448.30412.79.View ArticlePubMedGoogle Scholar
- D'Andrea V, Lippolis G, Biancari F, Ruco LP, Marzullo A, Wedard BM, Di Matteo FM, Sarmiento R, Dibra A, De Antoni E: A uterine pecoma: a case report. G Chir. 1999, 20: 163-164.PubMedGoogle Scholar
- Diment J, Colecchia M: Myomelanocytic tumor of the thigh. Am J Surg Pathol. 2003, 27: 1288-10.1097/00000478-200309000-00019.View ArticlePubMedGoogle Scholar
- Dimmler A, Seitz G, Hohenberger W, Kirchner T, Faller G: Late pulmonary metastases in uterine PEComa. J Clin Pathol. 2002, 56: 627-628. 10.1136/jcp.56.8.627.View ArticleGoogle Scholar
- Fukunaga M: Perivascular epithelioid cell tumor (PEComa) of soft tissue: case report with ultrastructural study. APMIS. 2004, 112: 98-104. 10.1111/j.1600-0463.2004.apm1120203.x.View ArticlePubMedGoogle Scholar
- Folpe AL: Neoplasms with perivascular epithelioid cell differentiation (PEComas). In: World Health Organization Classification of Tumors: Pathology and Genetics of Tumors of Soft Tissue and Bone. Edited by: Fletcher CDM, Unni KK, Mertens F. 2002, Lyon: IARC Press;, 221-222.Google Scholar
- Folpe AL, Goodman ZD, Ishak KG, Paulino AF, Taboada EM, Meehan SA, Weiss SW: Clear cell myomelanocytic tumor of the falciform ligament/ligamentum teres: a novel member of the perivascular epithelioid clear cell family of tumors with a predilection for children and young adults. Am J Surg Pathol. 2000, 24: 1239-46. 10.1097/00000478-200009000-00007.View ArticlePubMedGoogle Scholar
- Folpe AL, McKenney JK, Li Z, Smith SJ, Weiss SW: Clear cell myomelanocytic tumor of the thigh. Am J Surg Pathol. 2002, 26: 809-812. 10.1097/00000478-200206000-00018.View ArticlePubMedGoogle Scholar
- Gaffey MJ, Zarbo RJ, Weiss LM, Bonetti F, Pea M, Martignoni G, Zamboni G: PEC and Sugar. Am J Surg Pathol. 1992, 16: 307-308.PEC and sugar.,View ArticleGoogle Scholar
- Gaffey MJ, Mills SE: Clear cell tumor and angiomyolipoma [Letter]. Am J Surg Pathol. 1991, 15: 201-202.View ArticleGoogle Scholar
- Gaffey MJ, Mills SE, Zarbo RJ, Weiss LM: Clear cell tumor of the lung. Immunohistochemical and ultrastructural evidence of melanogenesis. Am J Surg Pathol. 1991, 15: 644-653.View ArticlePubMedGoogle Scholar
- Greene LA, Mount SL, Schned AR, Cooper K: Recurrent perivascular epithelioid cell tumor of the uterus (PEComa): an immunohistochemical study and review of the literature. Gynecol Oncol. 2003, 90: 677-681. 10.1016/S0090-8258(03)00325-1.View ArticlePubMedGoogle Scholar
- Govender D, Sabaratnam RM, Essa AS: Clear cell "sugar" tumor of the breast. Am J Surg Pathol. 2002, 26: 670-675. 10.1097/00000478-200205000-00014.View ArticlePubMedGoogle Scholar
- Hendrickson MR, Tavassoli FA, Kempson RL, McCluggage WG, Haller U, Kubik-Huch RA: Mesenchymal tumors and related lesions. In: World Health Organization Classification of Tumors. Pathology and Genetics of Tumors of the Breast and Female Genital Organs. Edited by: Tavassoli FA, Devilee P. 2003, Lyon: IARC Press, 233-244.Google Scholar
- Lehman NL: Malignant PEComa of the skull base. Am J Surg Pathol. 2004, 28: 1230-1232. 10.1097/01.pas.0000128668.34934.81.View ArticlePubMedGoogle Scholar
- Kung M, Landa JF, Lubin J: Benign clear cell tumor ("sugar") tumor of the trachea. Cancer. 1984, 54: 517-551.View ArticlePubMedGoogle Scholar
- Manganaro L, Ballesio L, Angeli ML, Bertini L, Di Seri M: US CT and MR findings in pecoma metastases. A case report. Radiol Med (Torino). 2002, 103: 433-436.Google Scholar
- Michal M, Zamecnik M: Hyalinized uterine mesenchymal neoplasms with HMB-45 positive epithelioid cells: epithelioid leiomyomas or angiomyolipomas?. Int J Surg Pathol. 2000, 8: 323-328.View ArticlePubMedGoogle Scholar
- Nuciforo PG, O'Hara CD: Correspondence. Mod Pathol. 2002, 15: 87-88. 10.1038/modpathol.3880495. Re: Bonetti F, Martignoni G, Colato C, Manfrin E, Gambacorta M, Faleri M, Bacchi C, Sin VC, Wong NL, Coady M, Chan JK: Abdominopelvic sarcoma of perivascular epithelioid cells: report of four cases in young women, one with tuberous sclerosis. Mod Pathol 2001, 14:563–8 and Tazelaar HD, Batts KP, Srigley JR: Primary extrapulmonary sugar tumor (PEST): a report of four cases. Mod Pathol 2001, 14:615–22.View ArticlePubMedGoogle Scholar
- Pan CC, Yu IT, Yang AH, Chiang H: Clear cell myomelanocytic tumor of the urinary bladder. Am J Surg Pathol. 2003, 27: 689-692. 10.1097/00000478-200305000-00013.View ArticlePubMedGoogle Scholar
- Pan CC, Yang AH, Chiang H: Malignant perivascular epithelioid cell tumor involving the prostate. Arch Pathol Lab Med. 2003, 127: e96-e98.PubMedGoogle Scholar
- Panizo-Santos A, Sola I, De Alava E, Lozano MD, Idoate MA, Pardo FJ: Angiomyolipoma and PEComa are immunoreactive for MyoD1 in cell cytoplasmic staining pattern. Appl Immunohistochem Mol Morphol. 2003, 11: 156-160.View ArticlePubMedGoogle Scholar
- Panizo A, Sola JJ, de Alava E, Idoate M, Toledo G, Pardo J: Angiomyolipoma and PEComa are immunoreactive for MyoD1 in cell cytoplasmic staining pattern [Abstract]. Mod Pathol. 2003, 16: 165A-10.1038/modpathol.3880736.View ArticleGoogle Scholar
- Park SH, Ro JY, Kim HS, Lee ES: Perivascular epithelioid cell tumor of the uterus: immunohistochemical, ultrastructural and molecular study. Pathol Int. 2003, 53: 800-805. 10.1046/j.1440-1827.2003.01557.x.View ArticlePubMedGoogle Scholar
- Pea M, Martignoni G, Zamboni G, Bonetti F: Perivascular epithelioid cell. Am J Surg Pathol. 1996, 20: 1149-1153. 10.1097/00000478-199609000-00012.View ArticlePubMedGoogle Scholar
- Pea M, Bonneti F, Zamboni G, Martignoni G, Fiore-Donati L, Doglioni C: Clear cell tumor and angiomyolipoma. Am J Surg Pathol. 1991, 15: 199-202.View ArticlePubMedGoogle Scholar
- Ruco LP, Pilozzi E, Wedard BM, Marzullo A, D'Andrea V, De Antoni E, Silvestrini G, Bonetti F: Epithelioid lymphangioleiomyomatosis-like tumor of the uterus in a patient without tuberous sclerosis: a lesion mimicking epithelioid leiomyosarcoma. Histopathology. 1998, 33: 91-93. 10.1046/j.1365-2559.1998.0415g.x.View ArticlePubMedGoogle Scholar
- Silva EG, Deavers MT, Bodurka DC, Malpica A: Uterine epithelioid leiomyosarcomas with clear cells: reactivity with HMB-45 and the concept of PEComa. Am J Surg Pathol. 2004, 28: 244-249. 10.1097/00000478-200402000-00013.View ArticlePubMedGoogle Scholar
- Sola JJ, Gomez-Roman J, Panizo A, Idoate MA, Lozano MD, Val-Bernal JF, Pardo J: Poor correlation between IHC and RT-PCR detection of tyrosinase in tumors derived from perivascular epithelioid cells (PEC tumors) [Abstract]. Mod Pathol. 2003, 16: 172A-Google Scholar
- Tanaka Y, Ijiri R, Kato K, Kato Y, Nakatani Y, Hara M: HMB-45/Melan-A and smooth muscle actin-positive clear-cell epithelioid tumor arising in the ligament teres hepatis: additional example of clear cell "sugar" tumors. Am J Surg Pathol. 2000, 24: 1295-1299. 10.1097/00000478-200009000-00015.View ArticlePubMedGoogle Scholar
- Tazelaar HD, Batts KP, Srigley JR: Primary extrapulmonary sugar tumor (PEST): a report of four cases. Mod Pathol. 2001, 14: 615-622. 10.1038/modpathol.3880360.View ArticlePubMedGoogle Scholar
- Tazelaar HD, Batts KP: In Reply. Re: Nuciforo PG, O'Hara CD: Mod Pathol. 2002, 15: 87-88. Mod Pathol 2002, 15:89–90.Google Scholar
- Vang R, Kempson RL: Perivascular epithelioid cell tumor ("PEComa") of the uterus. A subset of HMB-45 positive epithelioid mesenchymal neoplasms with an uncertain relationship to pure smooth muscle tumors. Am J Surg Pathol. 2002, 26: 1-13. 10.1097/00000478-200201000-00001.View ArticlePubMedGoogle Scholar
- Yanai H, Matsuura H, Sonobe H, Shiozaki S, Kawabata K: Perivascular epithelioid cell tumor of the jejunum. Pathol Res Pract. 2003, 199: 47-50.View ArticlePubMedGoogle Scholar
- Zamboni G, Pea M, Martignoni G, Zancanaro C, Faccioli G, Gilioli E, Pederzoli P, Bonetti F: Clear cell 'sugar' tumor of the pancreas: a novel member of the family of lesions characterized by the presence of perivascular epithelioid cells. Am J Surg Pathol. 1996, 20: 722-730. 10.1097/00000478-199606000-00010.View ArticlePubMedGoogle Scholar
- Sadeghi S, Krigman H, Maluf H: Perivascular epithelioid clear cell tumor of the common bile duct. Am J Surg Pathol. 2004, 28: 1107-1110. 10.1097/01.pas.0000116831.81882.d0.View ArticlePubMedGoogle Scholar
- Bhalla R, Dail D, Kozlowski P, Corman J: Multiple bilateral perivascular epithelioid cell tumor (PEComa) of the kidneys. Can J Urol. 2004, 11: 2296-2298.PubMedGoogle Scholar
- Fink D, Mardsen DE, Edwards L, Camaris C, Hacker NF: Malignant perivascular epithelioid cell tumor (PEComa) arising in the broad ligament. Int J Gynecol Cancer. 2004, 14: 1036-1039. 10.1111/j.1048-891X.2004.014549.x.View ArticlePubMedGoogle Scholar
- Gao Z, Bhuiya T, Anderson A: Perivascular epithelioid cell tumour (PEComa) of the uterus associated with malignant neoplasm of the female genital tract. J Obstet Gynaecol. 2004, 24: 600-604. 10.1080/01443610410001722905.View ArticlePubMedGoogle Scholar
- Garcia TR, Mestre de Juan MJ: Angiomyolipoma of the liver and lung: a case explained by the presence of perivascular epithelioid cells. Pathol Res Pract. 2002, 198: 363-367.View ArticlePubMedGoogle Scholar
- Cummings RJ, O'Grady A, Sabah M, Kay E, Leader M: Comparative genomic hybridization: emphasis on use of formalin-fixed paraffin-embedded tissue. Curr Diagn Pathol. 2003, 9: 173-178. 10.1016/S0968-6053(02)00097-2.View ArticleGoogle Scholar
- Hui P, Riba A, Pejovic T, Johnson T, Baergen RN, Ward D: Comparative genomic hybridization study of placental site trophoblastic tumor: a report of 4 cases. Mod Pathol. 2004, 17: 248-251. 10.1038/modpathol.3800025.View ArticlePubMedGoogle Scholar
- Fadare O, Bifulco C, Carter D, Parkash V: Cartilaginous differentiation in peritoneal tissues: a report of two cases and a review of the literature. Mod Pathol. 2002, 15: 777-780. 10.1097/01.MP.0000017565.19341.63.View ArticlePubMedGoogle Scholar
- Robboy SJ, Scully RE: Ovarian teratoma with glial implants on the peritoneum: an analysis of 12 cases. Hum Pathol. 1970, 1: 643-653.View ArticlePubMedGoogle Scholar
- Rosai J: Peritoneum, retroperitoneum, and related structures. In: Ackerman's Surgical Pathology. Edited by: Rosai J. 1996, St Louis: Mosby, 2: 2135-2172. 8Google Scholar
- Hironaka M, Fukayama M: Regional proliferation of HMB-45-positive clear cells of the lung with lymphangioleiomyomatosis-like distribution, replacing the lobes with multiple cysts and a nodule. Am J Surg Pathol. 1999, 23: 1288-1293. 10.1097/00000478-199910000-00016.View ArticlePubMedGoogle Scholar
- Chuah KL, Tan PH: Multifocal micronodular pneumocyte hyperplasia, lymphangioleiomyomatosis and clear cell micronodules of the lung in a Chinese female patient with tuberous sclerosis. Pathology. 1998, 30: 242-246.View ArticlePubMedGoogle Scholar
- Chiodera PL, Pea M, Martignoni G, Bonetti F: Pulmonary lymphangioleiomyomatosis associated with miliariform 'sugar' tumor. Int J Surg Pathol. 1995, 2 (Suppl): 483-486.Google Scholar
- Pileri SA, Cavazza A, Schiavina M, Zompatori M, Pederzoli M, Goldfischer M, Sabattini E, Ascani S, Pasquinelli G, Bonetti F, Colby TV: Clear-cell proliferation of the lung with lymphangioleiomyomatosis-like change. Histopathology. 2004, 44: 156-163.View ArticlePubMedGoogle Scholar
- Henske EP: Metastasis of benign tumor cells in tuberous sclerosis complex. Genes Chromosomes Cancer. 2003, 38: 376-381. 10.1002/gcc.10252.View ArticlePubMedGoogle Scholar
- Silva EG, Deavers MT, Bodurka D, Malpica A: Uterine epithelioid leiomyosarcomas with clear cells or malignant PEComas? [Abstract]. Mod Pathol. 2003, 16: 211A-10.1097/01.MP.0000057236.96797.07.View ArticleGoogle Scholar
- Zamecnik M, Michal M: HMB-45+ hyalinized epithelioid tumor of the uterus is linked to epithelioid leiomyoma rather than to PEC-omas. Int J Surg Pathol. 2001, 9: 341-343.View ArticlePubMedGoogle Scholar
- Smolarek TA, Bejarano PA, Heffelfinger S, Menon AG: HMB-45 immunoreactivity is present in both uterine leiomyomas and normal myometrium [Abstract]. Mod Pathol. 1999, 12: 125-Google Scholar
- Williams ME, Gainey TW, Lamb T, Bach D, Kornstein MJ, Burks RT: HMB-45 immunoreactivity in uterine mesenchymal tumors [Abstract]. Mod Pathol. 2000, 13: 120-10.1038/modpathol.3880121.View ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.