Table 1 Immunohistochemistry: antibody specifications and results

Spindle cells

Epithelioid cells

HMB-45

HMB-45

1:200

None

DakoCytomation Carpinteria, CA, USA

+++

+++

Vimentin

V9

1:5

None

Ventana, Tucson AZ, USA

+++

+

Desmin

D33

1:250

Trypsin

Ventana

+++

+

Progesterone receptor (PR)

PGR 636

1:2

Steam¶

DakoCytomation

++

++

Melan-A

A103

1:40

Steam

DakoCytomation

+++

+++

Estrogen receptor (ER)

1D5

1:2

Steam

DakoCytomation

-

-

Caldesmon

H-CD

1:100

Steam

DakoCytomation

+

-

Smooth muscle actin (SMA)

1A4

Neat

None

Sigma, St Louis, MO, USA

+++

++

Keratin

AE1/AE3

1:1200

Trypsin

Chemicon Int, Temecula, CA

-

-

S100

Polyclonal

1:2

Pronase

DakoCytomation

+

-

Ki-67 (LI)^¶

MIB-1

1:300

Steam

DakoCytomation

+(<1%)

+(<1%)

CD68

PG-M1

1:4

Steam

DakoCytomation

-

-

CD117

Polyclonal

1:200

Steam

DakoCytomation

-

-

EMA

E29

1:1000

None

DakoCytomation

-

-

p53

D07

1:3200

Steam

DakoCytomation

-

-