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First case report of ensartinib in a patient with metastatic ALK rearranged lung cancer with ALK I1171N mutation: a case report
World Journal of Surgical Oncology volume 21, Article number: 74 (2023)
Abstract
Background
The acquired resistance to ALK tyrosine kinase inhibitors (TKIs) in ALK-rearranged NSCLC is associated with poor survival outcomes and poses distinct clinical challenges. It is essential to develop potential therapeutic strategies for overcoming resistance.
Case presentation
Here, we first report a female lung adenocarcinoma patient with an acquired ALK resistance mutation (ALK 11171N) who was treated with ensartinib. Her symptoms significantly improved after only 20 days, and with a side effect of mild rash. Follow-up images observed no further brain metastases after 3 months.
Conclusions
This treatment may provide a new therapeutic strategy for ALK TKIs resistant patients, especially in position 1171 of ALK exon20.
Introduction
Non-small cell lung cancer (NSCLC) remains the leading cause of death worldwide, with most patients being diagnosed at advanced stages of the disease. Alectinib is now one of the first-line treatment options for ALK-positive NSCLCs who have developed resistance to crizotinib, and most patients respond to alectinib with an objective response rate (ORR) of about 50% [1]. However, patients with ALK-positive inevitably relapse within a year or two of treatment due to various resistance mechanisms, of which ALK I1171N was found to be highly resistant to alectinib in previous reports [2]. In recent years, ensartinib has become a new first-line treatment option for patients with ALK-positive NSCLC, which shows potent inhibition of wild-type ALK and most common crizotinib-resistant mutations (such as F1174 and C1156Y, G1269A, L1196M, S1206R, and T1151) [3]. Notably, F1174 and C1156Y have acquired resistance mutations to second-generation ALK TKIs that have been clinically reported, suggesting that ensartinib may become a potential treatment for second-generation resistance to ALK [4]. In this case report, we report a lung adenocarcinomas patient harboring an acquired resistance mutation ALK I1171N who showed good responses with ensartinib.
Case presentation
In March 2021, a 35-year-old female nonsmoker presented in another hospital with a lump located in her left neck. Ultrasound showed multiple abnormal lymph nodes in the left neck and suprasternal fossa. PET/CT showed a malignant lesion in the left posterior mediastinum and multiple metastases in the lungs, liver, and bones. A percutaneous biopsy on the left neck indicated a pathological diagnosis of T4N3M1c (IVB) metastatic lung adenocarcinoma. Next-generation sequence (NGS) analysis of the patient was performed to seek potential therapeutic regimens. This analysis revealed an EML4-ALK fusion (level, tissue 13.9%). The patient started oral alectinib 600 mg twice daily since May 2021. The abdominal CT images showed liver metastases accompanying multiple lymph node metastases in the hilar region one month later (Fig. 1A). The chest CT images still showed a malignant lesion in the left posterior mediastinum and diffuse bone metastases in the thoracolumbar spine. After 3 months of treatment, the chest and abdominal CT images showed thoracolumbar and bilateral multiple rib bone metastases were basically the same as before, multiple lymph node metastases in mediastinum, retroperitoneum and liver were less and smaller. According to the investigator’s assessment, the patient achieved a partial response at the first radiologic re-evaluation performed (Fig. 1B). However, her disease progressed after 7 months. Multiple liver lesions were observed to be significantly increased and enlarged than before (Fig. 1C). A percutaneous liver biopsy indicated adenocarcinoma. The liver metastases lesion and ctDNA were performed by NGS analysis and detected EML4-ALK fusion (level, plasma 0.24%) again, accompanied by an acquired ALK resistance mutation (ALK I1171N) (level, tissue 12.26%) (Fig. 2). During the period of taking alectinib, adverse event (AE) of the patient was increased alanine aminotransferase concentrations. Until December 2021, the patient stop took alectinib. According to the clinical treatment guidelines, the patient then took ensartinib as the second-line treatment immediately post alectinib. Surprisingly, 20 days later, CT re-examination showed that the multiple tiny nodules in both lungs were roughly the same as before, ultrasound re-examination revealed that most of the multiple lesions in the liver were reduced compared to before (Fig. 1D). AEs of the patient primarily included rash and pruritus (grade I), these symptoms significantly improved after allergy treatment. The patient did not appear to have noticeable brain metastases until March 14, 2022 (Fig. 1E).
Discussion
Here we reported an EML4-ALK fusion NSCLC patient with an acquired resistance mutation ALK I1171N, who benefited from ensartinib. The patient’s disease progressed after a 7-month-treatment of alectinib, and ALK I1171N mutation, a resistance mutation, was detected in the liver metastases. After switching to ensartinib, most of multiple lesions in the liver were reduced. The patient’s condition is relatively stable so far, and we will continue to follow-up. To our knowledge, this is the first case reporting ensartinib as the second-line treatment for the ALK I1171N mutation. Ensartinib is active against a broad array of ALK mutations, including C1156Y, F1174, G1269A, I1171, L1152R/V, and even G1202R [4, 5], some of which are one of the resistance mechanisms after ceritinib or alectinib treatment [6]. Hence, for the patients who gained the above mutation sites after the treatment of ceritinib or alectinib, ensartinib can be used as a treatment option to overcome drug resistance. A review of studies on ALK inhibitors showed that half maximal inhibitory concentration (IC50) value of crizotinib on ALK activity was 4.5 nmol/L. In comparison, the IC50 value of ensartinib on ALK activity was < 0.4 nmol/L, which indicated that ensartinib has a stronger inhibitory effect [7]. In a phase II multicenter trial, 52% of patients with ALK-positive NSCLC had objective response (OR) after the treatment of ensartinib and the median progression-free survival (PFS) was 9.6 months [5]. A phase I/II multicenter study showed that ensartinib also has a strong inhibitory effect on ALK tyrosine kinase with specific mutation sites, including F1174 and C1156Y, and their IC50 value was both less than 0.4 nmol/L; the response rate (RR) was 60% and median PFS was 9.2 months among the ALK-positive evaluable patients, the median duration of response (DOR) for ALK-positive evaluable patients at data cut-off was 12.8 months [6]. Moreover, the ORR of patients with I1171 mutation was relatively lower than other ALK alterations [6, 8]. In addition, gastrointestinal (e.g., diarrhea and vomiting), ocular, and cardiac toxic effects were less frequent with ensartinib; therefore, these results indicated that ensartinib has relatively good safety [3]. Furthermore, ensartinib showed superior systemic, especially intracranial efficacy, which is essential considering that the central nervous system is a common disease progression [6]. Consistent with the above results, our case did not experience obvious adverse effects and developed brain metastases. However, further clinical trials are needed to validate the effect of ensartinib on ALK I1171N in advanced NSCLCs.
Conclusions
In summary, our case report expands new treatment strategies for resistance to ALK TKIs, particularly the types of position 1171 of ALK exon20. Long-term follow-up of this patient and the evaluation of the efficacy of ensartinib will be continued. Furthermore, we will also pay close attention to whether the patient develops ensartinib resistance in the future.
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Acknowledgements
The authors thank Shanghai Tongshu Biotechnology Co., Ltd. for technical support and the patients and their family for their interest and cooperation.
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Contributions
Shuang Yang: data collection, writing-original draft preparation. Weineng Feng: data procession, writing—original draft preparation. Yanming Deng: software, validation. Jianmiao Liang: supervision, writing-reviewing, and editing. The author(s) read and approved the final manuscript.
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The study was approved by the ethics committee of First People’s Hospital of Foshan. Written informed consent was obtained from the participants for publication of the details of their medical case and any accompanying images.
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Informed consent has been obtained from the patient included in this study.
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The authors declare no competing interests.
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Yang, S., Feng, W., Deng, Y. et al. First case report of ensartinib in a patient with metastatic ALK rearranged lung cancer with ALK I1171N mutation: a case report. World J Surg Onc 21, 74 (2023). https://doi.org/10.1186/s12957-023-02935-9
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DOI: https://doi.org/10.1186/s12957-023-02935-9