Fig. 2

Knockdown efficiency of TMPO by lentivirus infection in the GBM cells. a Quantitative real-time PCR analysis of TMPO knockdown efficiency in U251 and U87 cells. The mRNA expression of TMPO was significantly suppressed when the cells were infected with TMPO-shRNA. b Western blot analysis validated TMPO-shRNA effectively downregulated the protein level of TMPO in U251 and U87 cells. c Western blot analysis confirmed TMPO-shRNA(S2) effectively decreased the protein expression of TMPO in U251 cells. **p < 0.01; scale bars, 10 μm