- Open Access
Gastric biomarkers: a global review
© The Author(s). 2016
Received: 7 January 2016
Accepted: 2 August 2016
Published: 11 August 2016
Gastric cancer is an aggressive disease with a poor 5-year survival and large global burden of disease. The disease is biologically and genetically heterogeneous with a poorly understood carcinogenesis at the molecular level. Despite the many prognostic, predictive, and therapeutic biomarkers investigated to date, gastric cancer continues to be detected at an advanced stage with resultant poor clinical outcomes.
This is a global review of gastric biomarkers with an emphasis on HER2, E-cadherin, fibroblast growth factor receptor, mammalian target of rapamycin, and hepatocyte growth factor receptor as well as sections on microRNAs, long noncoding RNAs, matrix metalloproteinases, PD-L1, TP53, and microsatellite instability.
A deeper understanding of the pathogenesis and biological features of gastric cancer, including the identification and characterization of diagnostic, prognostic, predictive, and therapeutic biomarkers, hopefully will provide improved clinical outcomes.
Gastric cancer (GC) has been globally the fourth most commonly diagnosed cancer and the second most lethal malignancy [1, 2]. The data has recently been changing, with WHO GLOBOCAN now reporting GC as the fifth most common cancer and third leading cause of cancer death in both sexes . In 2015, an estimated 24,590 new GC cases and 10,720 GC deaths were diagnosed in the USA . As most patients present with advanced unresectable or metastatic disease at the time of diagnosis, the overall clinical outcome of GC patients remains unsatisfactory, with a 5-year survival rate of less than 30 % [4–6]. The incidence of GC remains high in Japan, but the survival is higher, reported as 52 % [5, 6]. Clinicopathological staging using the TNM system is the major tool used by clinicians to predict GC patient prognosis. However, GC patients of identical TNM stage often exhibit varying clinical outcomes, suggesting that there are additional factors that influence long-term outcomes .
GC is a biologically heterogeneous disease that evolves in the background of various genetic and epigenetic alterations. Therefore, it is essential to have a more comprehensive understanding of molecular variables that affect GC disease pathways in order to develop appropriate approaches for its diagnosis and treatment . GC is assumed to originate from a sequential accumulation of molecular and genetic alterations to stomach epithelial cells , but the mechanism of carcinogenesis remains complex and poorly understood [9, 10]. Additionally, a number of cellular phenomena, such as tumour microenvironment, inflammation, oxidative stress, and hypoxia, act in parallel with various molecular events to promote initiation, progression, and metastasis of GC .
In the traditional Laurén classification, GC is divided into two types: intestinal and diffuse types [12, 13]. The intestinal-type adenocarcinomas characteristically form glands, but with various degrees of differentiation. Intestinal carcinomas are usually diagnosed in older patients, mostly in the antrum, and are strongly attributed to chronic H. pylori infection, with resultant atrophic gastritis, and intestinal metaplasia [12, 13]. Diffuse gastric carcinomas are poorly cohesive, composed of mostly single, or small, nests of neoplastic cells that diffusely infiltrate the gastric wall. This type is found most commonly in the gastric body and in younger patients. Although this type is also associated with H. pylori infection, the carcinogenetic sequence of the diffuse type of GC is not well characterized .
Features of gastric cancer sub-types defined by TCGA. Based on 295 patients (182 males, 113 females) 
• 43.0 % antrum
• 49.1 % fundus
• 64.9 % GEJ/cardia 50.0 % NA
• 26.1 % diffuse
• 60.2 % intestinal
• 52.6 % mixed
• 9.1 % non-specified
• 53.3 % M
• 44.2 % F
• TP53 mutation
• RTK-RAS activation
• 5.3 % antrum
• 13.8 % fundus
• 7.0 % GEJ/cardia
• 7.2 % diffuse
• 7.7 % intestinal
• 15.8 % mixed
• 27.3 % not specified
• 11.5 % M
• 4.4 % F
• PIK3CA mutation
• PD-L1/2 overexpression
• CDKN2A silencing
• 27.2 % antrum
• 21.6 % fundus
• 8.8 % GEJ/cardia
• 37.5 % NA
• 8.7 % diffuse
• 24.5 % intestinal
• 15.8 % mixed
• 63.6 % not specified
• 15.4 % M
• 31.9 % F
• MLH1 silencing
• Gastric CIMP
• 24.6 % antrum
• 15.5 % fundus
• 19.3 % GEJ/cardia 12.5 % NA
• 58.0 % diffuse
• 7.7 % intestinal
• 15.8 % mixed
• 19.8 % M
• 19.5 % F
• CDH1 mutations
• RHOA mutations
• CLDN18-ARHGAP fusion
Frequency of co-mutations in gastric cancer 
0/116 (0 %)
2/116 (1.7 %)
1/116 (0.9 %)
7/116 (6.0 %)
2/116 (1.7 %)
2/116 (1.7 %)
1/116 (0.9 %)
10/116 (8.6 %)
0/116 (0 %)
0/116 (0 %)
6/116 (5.2 %)
0/116 (0 %)
2/116 (1.7 %)
1/116 (0.9 %)
3/116 (2.6 %)
HER2 (encoded by ERBB2, the v-erb-b2 avian erythroblastic leukaemia viral oncogene homolog 2) is one of the four members of the human EGFR family (EGFR or HER1, HER2, HER3, and HER4) in the receptor tyrosine kinase (RTK) superfamily . Unlike other HER family members, HER2 does not contain a ligand-binding site and signals through hetero-dimerization with other HER family members, primarily EGFR . HER2 is expressed by normal and cancerous cells, whose gene amplification results in protein overexpression, subsequent cell proliferation, growth, and cell survival by triggering downstream signalling via the PI3K-AKT and the MAPK pathways [20, 21]. Although the prevalence, prognostic, and predictive value of HER2 is less established in GC compared to breast cancer, the importance is becoming evident as studies emerge.
Choice of study specimen. Some studies have used tissue microarray (TMA) [27, 29, 30], as opposed to whole slide for evaluation, and/or biopsy specimens as opposed to surgical specimens, which could be subject to sampling error due to tumour heterogeneity .
Methodology. Different in situ hybridization (ISH) techniques provide similar results, but interpretation is easier with silver-enhanced ISH (SISH), which is an advantage when diagnosing focal amplification in very small biopsies [32–34].
Biological. Relates to the intratumoural heterogeneity of HER2 alterations in GC, which has been shown to be of prognostic significance and is the main reason for discordance between IHC and FISH and between biopsies and resection specimens [35–49].
Location. The HER2 overexpression/amplification rate is higher in tumours from the gastroesophageal junction than in those located in more distal parts of the stomach . One study found a rate of HER2 expression in the distal stomach to be 32 % .
Scoring. In contrast to breast cancer, HER2 immunohistochemical expression in GC is more heterogeneous (focal staining) and may exhibit incomplete membrane staining. Therefore, an HER2 scoring system specific for GC has been developed with separate scoring systems for endoscopic biopsies and surgical resection specimens to ensure standardization .
The role of HER2 overexpression in tumourigenesis is not completely understood and its prognostic implication remains unclear . HER2 overexpression seems to be an early event in gastric carcinogenesis as HER2 expression rises significantly from low-grade to high-grade dysplasia to adenocarcinoma . Furthermore, the occurrence of HER2 expression in the early stage strongly suggests that there is no relationship between HER2 expression and prognosis . Indeed, some reports show no difference in prognosis when compared with HER2-negative tumours [22, 25–27, 30, 49, 51–54]. However, some studies do report that HER2 amplification is associated with a poor prognosis and aggressive disease [21, 29, 55–60]. For example, a systematic review investigating the prognostic value of HER2 overexpression found that 20 studies (57 %) reported no difference in overall survival (OS), 2 (6 %) showed significantly longer OS, and 13 (37 %) significantly worse OS . Interpretation of these controversial results is difficult due to lack of standardization in defining HER2 overexpression or amplification .
The relationships between HER2 status and other known pathologic and prognostic characteristics are more ambiguous. Some studies describe associations between HER2-positive tumours and nodal disease [30, 47], tumour size , depth of tumour invasion , and stage , whereas others fail to show any association [22, 56, 59]. Some reports have found no correlation between HER2 positivity and TNM stage of disease [38, 44, 46, 48]. Another study was unable to detect significant relationships between clinicopathologic factors and HER2 status with the exception that HER2-positive tumours demonstrated a lower prevalence of signet ring cell features . One other study noted that 100 % of tumour samples with signet ring cell features were HER2 negative . Since most studies failed to adjust for other confounders, it is difficult to interpret the reported relationship between HER2 and other histopathological variables.
HER2 expression has become the biomarker for identifying patients who are likely to show a survival benefit with trastuzumab . Trastuzumab, an HER2-targeted agent, has considerable activity in HER2-positive GC but only benefits patients with HER2-overexpressing/amplified tumours . The integration of targeted therapies in GC has been slower than in some other solid tumours , and trastuzumab is the only targeted agent approved for the treatment of advanced GC . At the biomolecular level, the HER2 pathway is responsible for the repair of DNA damage (particularly, inter-strand cross-links induced by platinum analogues), so that HER2-targeted inhibition may synergize with chemotherapy and increase apoptotic stress . The ToGA phase III international study assessed the efficacy in 594 patients with HER2-positive (IHC3+ or FISH+) advanced gastric or gastroesophageal junction cancer with a combination of trastuzumab + conventional chemotherapy as a treatment for GC patients. The trial demonstrated that advanced CG patients, stratified by HER2 amplification/overexpression, had longer median OS when treated with trastuzumab + chemotherapy versus chemotherapy alone (13.8 versus 11.1 months) . Targeting the extracellular domain of HER2 is not the end of the story; new ways of blocking this signalling pathway are being pursued.
Lapatinib is a dual EGFR/HER2 reversible tyrosine kinase inhibitor (blocking both HER1 and HER2) that suppresses the downstream signalling involving MAPK/Erk1/2 and PI3K/Akt pathways. The efficacy of lapatinib in conjunction with paclitaxel was assessed in a randomized phase III TyTAN trial, in Asian patients with HER2-positive advanced GC. The patients who progressed on first-line therapy were randomized to lapatinib in conjunction with weekly paclitaxel versus weekly paclitaxel alone. Median OS was 11.0 months with lapatinib plus paclitaxel versus 8.9 months with paclitaxel alone (p = 0.10), with no significant difference in median progression free survival (PFS) (5.4 versus 4.4 months) or time to progression (5.5 versus 4.4 months). Response rate was higher with lapatinib plus paclitaxel versus paclitaxel alone (odds ratio, 3.85; p < .001). However, the risk of death or disease progression was significantly lower in patients with IHC 3+ tumours who were treated with lapatinib, compared with those with IHC 0/1 or IHC 2+ tumours . Ado-trastuzumab emtansine (T-DM1) is an antibody-drug conjugate consisting of an antimicrotubule cytotoxic agent DM1 linked to trastuzumab. The phase II/III Gatsby trial evaluated efficacy of ado-trastuzumab emtansine in the second-line setting for the treatment of HER2-positive advanced GC. A total of 412 patients treated with first-line therapy participated in the study. The ImmunoGen, Inc., has recently disclosed that the trial did not meet its primary endpoint of OS. The trial findings have not presented yet .
Other HER2-directed therapies such as pertuzumab and neratinib that have demonstrated efficacy in breast cancer have not yet been evaluated in randomized clinical trials in patients with HER2-positive GC. Furthermore, dual HER2 blockade, which is an effective strategy in breast cancer, is being investigated in a phase II study using the combination of pertuzumab and trastuzumab in patients with HER2-positive metastatic GC. HER2 is a promising biomarker for targeted treatment in GC. Several clinical trials are currently exploring HER2-directed therapy in patients with GC using varied designs. The results of these future studies will be helpful to know the efficacy and tolerance of HER2-directed therapy in HER2-positive GC.
CDH1, located on chromosome 16 (q 22.1), encodes the E-cadherin transmembrane protein [66, 67]. E-cadherin is a calcium-mediated membrane molecule that plays an important role in adhesion and differentiation of gastric epithelial cells, which is a very important protective mechanism against neoplasm formation . E-cadherin is one of the most important tumour suppressor genes in GC, and its inactivation is thought to contribute to tumour progression via subsequent increases in proliferation, invasion, and metastasis [15, 66, 68–72]. E-cadherin dysfunction may occur through several mechanisms, including CDH1 mutations, epigenetic silencing by promoter hypermethylation, loss of heterozygosity (LOH), transcriptional silencing by a variety of transcriptional repressors that target the CDH1 promoter, and microRNAs that regulate E-cadherin expression . However, only the presence of E-cadherin structural alterations represents a poor prognostic factor . E-cadherin somatic alterations exist in all clinical settings and histotypes of GC and are associated with different survival rates . These alterations are, presently, non-targetable as this would require restoring E-cadherin expression by gene therapy . Nevertheless, E-cadherin is a potential predictive marker of response to therapy since its impairment decreases tumour cell sensitivity to conventional and targeted therapies [72, 73]. Screening for CDH1 mutations at the time of GC diagnosis may help to predict patient prognosis and is likely to improve management of patients .
Multiple germline E-cadherin mutations have been reported in hereditary diffuse gastric cancer (HDGC) . Analysis of families demonstrated an association between GC development and germline mutations in the E-cadherin (CDH1) gene. The CDH1 gene mutations have been scattered across the 16 exons this gene encompasses, with approximately 75 % being truncating and 25 % missense in nature [75, 76]. Moreover, there have even been large deletions of the E-cadherin gene identified in a small percentage (4 %) of HDGC families, likely involving nonallelic homologous recombination in Alu repeat regions . Furthermore, 70 % of CDH1 mutation-negative HDGC probands display germline monoallelic CDH1 RNA downregulation (allelic imbalance), reinforcing the role of the CDH1 locus in this disease . HDGC tumours appear when complete somatic CDH1 inactivation is acquired, leading to reduced or absent E-cadherin expression [75, 79]. This occurs through second-hit mechanisms, pursuing Knudson’s model of tumour suppressor gene inactivation [80, 81]. CDH1 promoter hypermethylation is the most frequent second-hit inactivation mechanism in HDGC primary tumours, whereas a second mutation or deletion (LOH/intragenic deletions) is less frequently identified [70, 82–84]. The cumulative risk estimate for advanced GC by 80 years of age was estimated to be 67 % for men and 83 % in women with wide confidence intervals, as these were based on 11 HDGC families .
A study of 42 families diagnosed with HDGC trait by having at least two members affected had an E-cadherin mutation identified in 40 % of cases. If the clinical criteria were less stringent to include only one GC occurring before 50 years of age, then more than half the cases had E-cadherin mutations . When large deletions were screened in addition to point mutations and small frameshift mutations, 46 % of 160 high-risk families were found to have a germline E-cadherin gene alteration . It is noteworthy that more than half to up to two thirds of HDGC families reported have proven negative for the E-cadherin gene mutation [77, 87]. Allele expression imbalance of CDH1 was noted in a subset of these families ; however, most of these families likely have other molecular alterations underlying their cancer predisposition that are yet to be discovered . The frequency of abnormal E-cadherin expression among sporadic DGC varies and has been reported as 7 , 38 , 46 , and 82 % . CDH1 somatic alterations were found in approximately 30 % of all patients with GC . No germline mutations of this gene were detected in apparent sporadic diffuse GC cases with a mean age of 62 years in Great Britain . Furthermore, a study of 25 sporadic diffuse GCs identified 1 case with a germline E-cadherin mutation and none in 14 intestinal-type GCs . Molecular variables such as CDH1 alterations may be crucial to better define the survival of patients with a family history.
Abnormal E-cadherin expression may be used as a predictive factor for tumour invasiveness in gastric adenocarcinoma. One study showed a significant correlation between abnormal E-cadherin expression and tumour grade and regional lymph node involvement . Another study showed that E-cadherin methylation was correlated with size of tumour, tumour stage, and nodal metastases . However, one study found that E-cadherin mutation was not correlated with tumour grade or stage . In keeping with most studies finding a correlation of abnormal E-cadherin expression with adverse clinicopathologic factors, tumours with CDH1 structural alterations displayed a significantly poorer survival rate than tumours negative for CDHI alterations or tumours with epigenetic CDH1 alterations . For instance, one study found that patients with GC displaying CDH1 exon 8/9 deletions (structural) have a worse clinical evolution and a shorter OS . Overall, abnormal E-cadherin expression favours a worse prognosis for GC patients.
Important biomarkers in GC currently being investigated include the FGFR, the hepatocyte growth factor receptor (HGFR, MET), and mTOR .
FGFR family members (FGFR1, FGFR2, FGFR3, and FGFR4) belong to the RTK superfamily . In a recent genomic survey of GC using high-resolution single-nucleotide polymorphism (SNP) arrays, FGFR2 copy number gain was found in 9 % of tumours and was more common than EGFR (8 %), HER2 (7 %), or MET (4 %) copy number gains . FGFR2 has therefore attracted significant attention as a potential candidate for targeted therapy in GC . The small-molecule FGFR2 inhibitor, dovitinib (TKI258), has demonstrated growth inhibitory activity in FGFR2-amplified GC cell lines and xenografts. Ongoing phase II studies will be helpful to clarify the role of dovitinib in patients with FGFR-amplified metastatic GC .
Phosphatidylinositol-3-kinase (PI3K)/mTOR represents one common final convergence signalling pathway originated by the activation of several RTKs. Oncogenic mutations in PIK3CA (gene encoding the alpha p110 catalytic subunit) of PI3K have been observed in GC, constitutively activating the PI3KA/mTOR pathway [15, 98]. Studies in GC have reported a mutation frequency ranging from 5 to 67 % [99–102]. In particular, EBV-positive tumours have a strong predilection for PIK3CA mutations . Misregulation has been associated with increased lymph node metastases and decreased survival of GC patients [15, 103, 104]. Everolimus is an mTOR inhibitor that has shown potential benefit in advanced GC in early phase 2 trials [105, 106]. A phase 3 trial compared everolimus with placebo in 656 patients with chemotherapy refractory advanced GC . Only a trivial improvement in PFS was noted (median 1.7 versus 1.4 months; p < 0.001). There was no significant improvement in OS (median 5.4 versus 4.3 months; p = 0.124).
EGFR, another member of the human tyrosine kinase receptor family, has been shown to be overexpressed by IHC in 27 % of GCs, whereas gene amplification by FISH was evident in less than 3 % in one large series . Another series using FISH found an incidence of EGFR amplification to be ~5 % . EGFR amplification was found to be 8 % using SNP assays . EGFR overexpression has been associated with the presence of moderately or poorly differentiated histology, higher stage, and poor survival .
The addition of an anti-EGFR monoclonal antibody with cytotoxic chemotherapy, however, has failed to demonstrate improvement in the outcomes of patients with advanced GC. Unlike HER2 target-directed therapy, there are no established biomarkers to predict response to EGFR inhibitors. The predictive value of EGFR mutation, increased EGFR copy number, and K-ras mutation status in GC remains controversial. Two randomized clinical trials “EXPAND” and “REAL3” evaluated efficacy of an anti-EGFR monoclonal antibody (panitumumab or cetuximab) in combination with chemotherapy in patients with advanced gastric and oesophageal cancer [110, 111]. In the EXPAND trial, 904 patients with advanced GC were randomized to capecitabine and cisplatin plus cetuximab or chemotherapy alone. The median PFS of patients who received chemotherapy plus cetuximab was 4.4 months compared with 5.6 months with chemotherapy alone . In the REAL3 trial, 553 patients with advanced gastric and oesophageal cancer were randomized to chemotherapy plus panitumumab or chemotherapy alone. Median OS of patients who received chemotherapy was 11.3 months compared with 8.8 months if they received chemotherapy plus panitumumab (p = 0.013) . In addition to anti-EGFR monoclonal antibodies, orally active tyrosine kinase inhibitors (TKIs), small molecules that block the binding site of the EGFR tyrosine kinase, have been evaluated in patients with chemotherapy refractory advanced gastric and oesophageal cancer. In a phase II trial of 70 patients, erlotinib monotherapy resulted in a response rate of 9 % in patients with gastroesophageal junction cancer but none in the GC subgroup . At the present time, anti-EGFR therapies do not add to conventional chemotherapy. There is a need for further investigations to identify subset of patients who will benefit from EGFR blockade.
MET (encoded by MET) belongs to the HGFR family . MET is a transmembrane tyrosine kinase receptor with high affinity for hepatocyte growth factor/scatter factor (HGF/SF). Auto-phosphorylation of MET activates several signalling transduction cascades, leading to cancer cell proliferation, angiogenesis, invasion, and metastases .
MET amplification and/or overexpression of its protein product has long been implicated in the pathogenesis of GC, with many reports based on gene copy number, RNA expression, and/or protein expression, supporting its role as a poor prognostic factor [114–117]. Nevertheless, the prevalence of MET amplification in GC varies widely in the literature from 0  to 68 % [113, 115]. This discrepancy is greatly attributed to the methodology employed to detect gene amplification/copy number gain and/or protein expression . MET-positive tumours were more frequently associated with serosal invasion and other unfavourable features . In all studies [97, 115–117], the GC patients with polysomic and/or amplified MET showed poorer disease-free survival and OS in comparison with the non-polysomic MET . Another study has reported a significantly worse prognosis for MET-positive compared with MET-negative tumours . Although MET amplification may play a central role in determining GC prognosis, future studies should focus on the possible negative predictive role for response to chemotherapy or targeted therapies .
Despite the fact that aberrant up-regulation of the MET/HGF pathway is associated with poor prognosis in GC, anti-MET therapies have shown limited efficacy in advanced GC. Onartuzumab is a fully humanized, mono-valent anti-MET antibody that inhibits HGF binding and receptor activation. The efficacy of onartuzumab in combination with chemotherapy (mFOLFOX6) in the first-line setting was examined for metastatic, HER2-negative gastroesophageal cancer . In the MET-positive subgroup, median PFS was 5.95 months for onartuzumab and 6.8 months for placebo (HR 1.38 [0.60–3.20]). Likewise, the interim results of a phase II study of foretinib, a MET TKI, showed minimal activity in a MET-unselected patient cohort . Despite early negative results, several novel MET inhibitors are now being evaluated in metastatic or unresectable GC in an attempt to identify patients who respond to MET inhibitors.
Programmed death-1 (PD-1) is a key immune checkpoint receptor critical for the regulation of T cell function during immunity and tolerance. The PD-1 surface receptor binds to two ligands, PD-L1 and PD-L2, which are expressed on tumour cells. PD-1-PD-L interactions control the induction and maintenance of peripheral T cell tolerance. Tumours use the PD-1 pathway to evade immune surveillance and to prevent the immune system from rejecting the tumour . The frequency of programmed death ligand 1 (PD-L1) overexpression, a putative response biomarker, approaches 40 % in GC . The EBV-positive sub-type of tumours has shown increased expression of PD-L1/2 . Pembrolizumab is an anti-PD1 monoclonal antibody that has shown efficacy in advanced PD-1-expressing GC. In an early phase trial, 65 patients who had distinctive stromal or ≥1 % tumour nest cell PD-L1 staining were treated with pembrolizumab. The objective response rate was 22 %, and median response duration was 24 weeks. The 6-month PFS was 24 % and the 6-month OS was 69 % . The results of this study have prompted expansion of immune checkpoint inhibitors in advanced GCs. Targeting the PD-1 pathway and immune checkpoint blockade appears to be a promising novel approach for the treatment of GC.
The TP53 gene encodes a nuclear p53 protein of 393 amino acids, which acts as a potent transcription factor with a key role in the maintenance of genetic stability [125, 126]. The function of TP53 gene is usually altered through LOH, mutations, and rarely by DNA methylation . TP53 mutation is one of the most prevalent genetic alterations in GC and associated with the CIN sub-type of GC [14, 126]. More than one mutation may be present in a single tumour resulting in heterogeneity of the TP53 mutational status .
There are conflicting results with respect to the prevalence of TP53 mutations and their relationship to histological type or tumour stage of GC. Some studies showed that mutations tend to affect mainly intestinal-type tumours, while others found that the incidence of mutation is similar in both intestinal and diffuse-type tumours, ranging between 16 and 65 % of the cases studied. The frequency of TP53 abnormalities in both early and advanced intestinal type is consistent, similar to that observed in advanced diffuse type, while in early diffuse-type TP53 mutations are uncommon [126–130].
The expression of p53 in non-tumour gastric mucosa with dysplasia was significantly higher than that in the mucosa without dysplasia. Overexpression of p53 protein was associated with the size of tumours that may help in diagnosis and prognostic prediction of GC . However, the prognostic impact of p53 abnormalities on this neoplasm remains controversial. A significant association between p53 overexpression and the metastatic spread to lymph nodes or shortened survival has been described by some studies on GC but not by others . At this time, p53 is not a reliable prognostic factor for GC.
Microsatellites are short iterations of 1–6 nucleotide long units, non-randomly distributed in both prokaryotic and eukaryotic genomes . Mismatch repair (MMR) deficiency leads to a tumour phenotype known as microsatellite instability (MSI), in which cells accumulate genetic errors . Several reports have shown the association of GC with MSI [132, 133]. MSI has been reported in 15 to 30 % of GC, mainly due to epigenetic silencing via hypermethylation of the MLH1 promoter [134, 135]. MSI-positive GC generally develops later in life and has a favourable prognosis when compared with MSI-negative tumours [136, 137]. The methylation of hMLH1 gene and its loss of expression increase with increasing age of the GC patient . Studies have shown a strong association of MSI in GC with intestinal type, which undergoes more genomic instability in comparison to the diffuse type . Moreover, MSI GC is more common in the distal part of the stomach [137, 139]. Interestingly, MSI tumours usually have an overall long-term prognosis that is favourable even in patients with advanced disease due to the fact that these tumours have a lower ability to invade serosal layers and are associated with a lower prevalence of lymph node metastases [135, 137, 139–141]. There are also higher survival rates in patients with advanced MSI GC in comparison to patients with other types of GC even with the same identical stage of the disease . Information is scarce as to the prognostic value of EGFR, HER2, or VEGFA expression in the MSI subset of GC . The MSI status certainly appears to be an independently positive prognostic factor, and future studies will need to determine the impact of MSI GC in the context of other co-existent molecular alterations.
MicroRNAs (miRNAs) are short fragments of noncoding RNAs comprising 18 to 24 ribonucleotides that can regulate the expression of genes by directly binding to the 3′UTR region of their target gene mRNA and impairing their translation [143, 144]. miRNAs have been found to regulate a variety of cellular processes such as cell proliferation, differentiation, invasion, migration, and epithelial-mesenchymal transition . A single miRNA can regulate the expressions of thousands of genes and participate in the regulation of the whole cell cycle . In detail, miRNAs negatively regulate the expression of cancer-related genes by decreasing the expression of tumour suppressor genes or enhancing the expression of oncogenes, or as modulators of cancer stem cells and metastases . Accumulating evidence suggests that miRNAs play an important role in GC, but the role of specific miRNAs involved in this disease remains elusive .
Serum and plasma miRNAs are more stable and relatively easier to access than tissue samples . The stability of tumour-associated miRNA in blood allows it to be a novel noninvasive tumour biomarker for cancer detection . Circulating miRNAs must demonstrate several hallmark characteristics to be considered as reliable biomarkers: (i) stable and able to be quantified in clinical samples; (ii) present at undetectable or low levels in samples from individuals without cancer, while being expressed by cancer cells at moderate or high levels; (iii) exhibit biological functions mechanistically linked to malignant tumour progression; and (iv) provide diagnostic or prognostic information [149, 150].
The results on expression of miRNAs are inconsistent, and it is hard to select a suitable miRNA as a cancer biomarker. Furthermore, there is no consensus regarding whether plasma or serum is preferable for use as a sample, and there is a limitation to analysing the miRNA expression results of both plasma and serum . Previous studies have shown that some miRNAs have been inconsistently reported when being used as a cancer biomarker [151, 152]. Possible reasons for these observed inconsistencies are the diverse experimental techniques, lack of sufficient relevant clinical data, the heterogeneous tissue samples, and poor study design [145, 153].
Numerous miRNAs have been identified as dysregulated in GC, either tissues or cell lines, many of which have also been associated with clinicopathologic features and/or survival. A number of reviews have specifically addressed the role of miRNAs in GC with comprehensive tables [154, 155]. However, to date there are no validated therapeutic trials showing miRNAs are an effective novel prognostic, predictive, or therapeutic biomarker.
Long noncoding RNAs
Long noncoding RNAs (lncRNAs) are functional RNAs longer than 200 nucleotides . According to the proximity to protein-coding genes, lncRNAs can be classified as sense, antisense, divergent or bidirectional, intronic, and intergenic . As entities of transcriptional control, generally it is understood that lncRNAs may perform their functions in at least two ways: (i) as scaffoldings in ribonucleoprotein complexes, e.g. transcription or chromatin-modifying factors, acting in cis or in trans on the genome , and (ii) as incidental by-products of a negative type of transcriptional regulation termed “transcriptional interference” . Unlike protein-coding genes, the function of these lncRNAs and their relevance to disease remain unclear . Recently, a new regulatory mechanism has been identified in which crosstalk between lncRNAs and mRNA occurs by competing for shared miRNAs response elements. In this case, lncRNAs may function as competing endogenous RNAs to sponge miRNAs, thereby modulating the de-repression of miRNA targets and imposing an additional level of post-transcriptional regulation .
LncRNAs are still an emerging field. However, accumulating evidence has demonstrated that many lncRNAs are dysregulated in GC and closely related to tumorigenesis, metastases, and prognosis or diagnosis . A total of 135 lncRNAs have been found to be aberrantly expressed in GC tissues [11, 161]. These may be potential prognostic biomarkers for GC and await future studies to further elucidate their relevance.
The matrix metalloproteinases (MMPs) are a family of 24 zinc-dependent endopeptidases in humans that degrade components of the extracellular membrane (ECM) . MMPs participate in several normal and pathological processes, and their activity is mainly modulated by the action of the tissue inhibitor of metalloproteinase (TIMP) . MMPs take part in breaking down the extracellular matrix in normal physiological processes . Specifically, it has been reported that both the expression of some MMP proteins and mRNA may have a large influence on GC [165, 166]. Studies regarding regulation of MMPs and TIMPs in GC have suggested that these molecules could be useful as markers of depth of invasion, metastases, and peritoneal dissemination . There are some conflicting results, which are most likely related to methodological aspects and to the heterogeneity of the patient populations .
MMPs have been identified as up-regulated in GC, either tissues or cell lines, and have also been associated with clinicopathologic features and/or survival including MMP 3 , 7 [168–170], 11 [162, 168], 9 [168, 171, 172], 12 , 21 [168, 173], MT1 [174–176], 14 , 1 , 2 , and 28 .
MMP inhibitors, however, have shown limited clinical benefit. For example, a randomized, double-blind, placebo-controlled study evaluated efficacy of orally administered MMP, marimastat, in 369 patients with chemotherapy refractory advanced gastric and gastroesophageal cancer. A modest difference in survival was noted. The median survival was 138 days for placebo and 160 days for marimastat, with a 2-year survival of 3 and 9 %, respectively. The treatment was complicated by poor tolerability and was associated with musculoskeletal pain and inflammation . Though many studies have identified the possible role of MMPs in GC, the clinical correlation is still lacking and many more studies will need to be carried out.
GC is common according to global estimates of cancer and is a frequent cause of cancer-related mortality with a poor survival. At present, although the role of many genetic alterations discovered in GC seems unclear, they represent a promising tool for stratifying patients according to tumour biological behaviour and likelihood of response to systemic therapy . Nevertheless, to date, except for HER2, there are no established evidence-based biomarkers predictive of tumour response to targeted agents, and the majority of patients do not yet benefit from molecularly directed therapies.
Most analyses in the literature consider a limited number of cases. As a result, despite the huge amount of data, no novel/reliable molecular marker has been introduced in the frame of secondary prevention strategies, so far . It is therefore important to undertake retrospective studies in which tumour samples from patients that have undergone GC therapy are mined for single or combinations of biomarkers that can predict favourable/unfavourable response towards a certain chemotherapeutic regimen or define the use of multiple therapy regimens in GC. Independent validation of the most promising prognostic and predictive biomarkers will then be required before they can be routinely employed in clinical practice . The search for a diagnostic biomarker is particularly critical for patient outcomes as early diagnosis would be instrumental in increasing survival. Future studies with identification and validation of diagnostic, prognostic, predictive, and therapeutic biomarkers will aid in the understanding of GC resulting in personalized pathway-driven targeted therapy with improved patient outcomes .
CIN, chromosomal instability; ECM, extracellular membrane; EGFR, human epidermal growth factor receptor family; FGFR, fibroblast growth factor receptor; GC, gastric cancer; GS, genomically stable; HDGC, hereditary diffuse gastric cancer; HGF/SF, hepatocyte growth factor/scatter factor; HGFR, MET, hepatocyte growth factor receptor; ISH, in situ hybridization; lncRNAs, long noncoding RNAs; LOH, loss of heterozygosity; miRNA, microRNA; MMPs, matrix metalloproteinases; MMR, mismatch repair; MSI, microsatellite instability; mTOR, mammalian target of rapamycin; PD-1, programmed death-1; PD-L1, programmed death ligand 1; RTK, receptor tyrosine kinase; SISH, silver-enhanced in situ hybridization; SNP, single-nucleotide polymorphism; TCGA, The Cancer Genome Atlas; TIMP, tissue inhibitor of metalloproteinase; TKIs, tyrosine kinase inhibitors; TMA, tissue microarray; ToGA, Trastuzumab for Gastric Cancer
Availability of data and materials
NB conducted the literature search, drafted the initial manuscript, critically reviewed the manuscript, and approved the final manuscript as submitted. J-LS conducted the literature search and approved the final manuscript as submitted. SA drafted the initial manuscript, critically reviewed the manuscript, and approved the final manuscript as submitted. SK critically reviewed the manuscript and approved the final manuscript as submitted. RK critically reviewed the manuscript and approved the final manuscript as submitted.
The authors declare that they have no competing interests.
Consent for publication
Ethics approval and consent to participate
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Jemal A, Bray F, Center MM, et al. Global cancer statistics. CA Cancer J Clin. 2011;61(2):69–90.View ArticlePubMedGoogle Scholar
- Nagini S. Carcinoma of the stomach: a review of epidemiology, pathogenesis, molecular genetics and chemoprevention. World J Gastrointest Oncol. 2012;4(7):156–69.PubMed CentralView ArticlePubMedGoogle Scholar
- Iarc . (2016). GLOBOCAN 2012: Estimated cancer incidence, mortality, and prevalence worldwide in 2012 . Retrieved 19 July, 2016, from http://globocan.iarc.fr/Pages/fact_sheets_cancer.aspx. Accessed 4 Aug 2016.
- Siegel RL, Miller KD, Jemal A. Cancer statistics. CA Cancer J Clin. 2015;65(2015):5–29.View ArticlePubMedGoogle Scholar
- Ohtsu A. Chemotherapy for metastatic gastric cancer: past, present, and future. J Gastroenterol. 2008;43:256–64.View ArticlePubMedGoogle Scholar
- Hartgrink HH, Jansen EP, van Grieken NC, et al. Gastric cancer. Lancet. 2009;374:477–90.PubMed CentralView ArticlePubMedGoogle Scholar
- Ahn JR, Jung M, Kim C, et al. Prognosis of pN3 stage gastric cancer. Cancer Res Treat. 2009;41:73–9.PubMed CentralView ArticlePubMedGoogle Scholar
- Yasui W, Sentani K, Sakamoto N, et al. Molecular pathology of gastric cancer: research and practice. Pathol Res Pract. 2011;207:608–12.View ArticlePubMedGoogle Scholar
- Nobili S, Bruno L, Landini I, et al. Genomic and genetic alterations influence the progression of gastric cancer. World J Gastroenterol. 2011;17:290–9.PubMed CentralView ArticlePubMedGoogle Scholar
- Sun M, Nie FQ, Wang ZX, et al. Involvement of lncRNA dysregulation in gastric cancer. Histol Histopathol. 2015;21:11655.Google Scholar
- Li PF, Chen SC, Xia T, et al. Non-coding RNAs and gastric cancer. World J Gastroenterol. 2014;20(18):5411–9.PubMed CentralView ArticlePubMedGoogle Scholar
- Shokal U, Sharma PC. Implication of microsatellite instability in human gastric cancers. Indian J Med Res. 2012;135(5):599–613.PubMed CentralPubMedGoogle Scholar
- Lauwers GY. Odze and Goldblum Surgical Pathology of the GI Tract, Liver, Biliary Tract and Pancreas (3rd Ed), Chapter 25, 707-721. Philadelphia: Elsevier, Saunders; 2015.Google Scholar
- Cancer Genome Atlas Research Network. Comprehensive molecular characterization of gastric adenocarcinoma. Nature. 2014;513:202–9.View ArticleGoogle Scholar
- Durães C, Almeida GM, Seruca R, et al. Biomarkers for gastric cancer: prognostic, predictive or targets of therapy? Virchows Arch. 2014;464(3):367–78.View ArticlePubMedGoogle Scholar
- Lin LL, Huang HC, Juan HF. Discovery of biomarkers for gastric cancer: a proteomics approach. J Proteomics. 2012;75(11):3081–97.View ArticlePubMedGoogle Scholar
- Italiano A. Prognostic or predictive? It’s time to get back to definitions! J Clin Oncol. 2011;29(35):4718.View ArticlePubMedGoogle Scholar
- Fareed KR, Kaye P, Soomro IN, et al. Biomarkers of response to therapy in oesophago-gastric cancer. Gut. 2009;58(1):127–43.View ArticlePubMedGoogle Scholar
- Ou SH. Second-generation irreversible epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (TKIs): a better mousetrap? A review of the clinical evidence. Crit Rev Oncol Hematol. 2012;83(3):407–21.View ArticlePubMedGoogle Scholar
- Gallardo A, Lerma E, Escuin D, et al. Increased signaling of EGFR and IGF1R, and deregulation of PTEN/PI3K/Akt pathway are related with trastuzumab resistance in HER2 breast carcinomas. Br J Cancer. 2012;106:1367–73.PubMed CentralView ArticlePubMedGoogle Scholar
- Gravalos C, Jimeno A. HER2 in gastric cancer: a new prognostic factor and a novel therapeutic target. Ann Oncol. 2008;19:1523–9.View ArticlePubMedGoogle Scholar
- Grabsch H, Sivakumar S, Gray S, et al. HER2 expression in gastric cancer: rare, heterogeneous and of no prognostic value—conclusions from 924 cases of two independent series. Cell Oncol. 2010;32:57–65.PubMed CentralPubMedGoogle Scholar
- Allgayer H, Babic R, Gruetzner KU, et al. c‐erbB‐2 is of independent prognostic relevance in gastric cancer and is associated with the expression of tumor‐associated protease systems. J Clin Oncol. 2000;18:2201–9.PubMedGoogle Scholar
- Okines AF, Cunningham D. Trastuzumab in gastric cancer. Eur J Cancer. 2010;46:1949–59.View ArticlePubMedGoogle Scholar
- Chan DS, Campbell F, Edwards P, et al. Relative prognostic value of human epidermal growth factor receptor 2 (HER2) expression in operable oesophagogastric cancer. ISRN Surg. 2012;2012:804891.PubMed CentralView ArticlePubMedGoogle Scholar
- Phillips BE, Tubbs RR, Rice TW, et al. Clinicopathologic features and treatment outcomes of patients with human epidermal growth factor receptor 2‐positive adenocarcinoma of the esophagus and gastroesophageal junction. Dis Esophagus. 2013;26:299–304.View ArticlePubMedGoogle Scholar
- Okines AF, Thompson LC, Cunningham D, et al. Effect of HER2 on prognosis and benefit from peri‐operative chemotherapy in early oesophago‐gastric adenocarcinoma in the MAGIC trial. Ann Oncol. 2013;24:1253–61.View ArticlePubMedGoogle Scholar
- Bang YJ, Van Cutsem E, Feyereislova A, et al. Trastuzumab in combination with chemotherapy versus chemotherapy alone for treatment of HER2-positive advanced gastric or gastro-oesophageal junction cancer (ToGA): a phase 3, open-label, randomised controlled trial. Lancet. 2010;376(9742):687–97.View ArticlePubMedGoogle Scholar
- Yan B, Yau EX, Bte Omar SS, et al. A study of HER2 gene amplification and protein expression in gastric cancer. J Clin Pathol. 2010;63:839–42.View ArticlePubMedGoogle Scholar
- Zhou F, Li N, Jiang W, et al. Prognosis significance of HER-2/neu overexpression/amplification in Chinese patients with curatively resected gastric cancer after the ToGA clinical trial. World J Surg Oncol. 2012;10:274.PubMed CentralView ArticlePubMedGoogle Scholar
- Warneke VS, Behrens HM, Boger C, et al. Her2/neu testing in gastric cancer: evaluating the risk of sampling errors. Ann Oncol. 2013;24:725–33.View ArticlePubMedGoogle Scholar
- Boers JE, Meeuwissen H, Methorst N. HER2 status in gastro-oesophageal adenocarcinomas assessed by two rabbit monoclonal antibodies (SP3 and 4B5) and two in situ hybridization methods (FISH and SISH). Histopathology. 2011;58(3):383–94.PubMed CentralView ArticlePubMedGoogle Scholar
- Rüschoff J, Hanna W, Bilous M, et al. HER2 testing in gastric cancer: a practical approach. Mod Pathol. 2012;25(5):637–50.View ArticlePubMedGoogle Scholar
- Yan B, Yau EX, Choo SN, et al. Dual-colour HER2/chromosome 17 chromogenic in situ hybridisation assay enables accurate assessment of HER2 genomic status in gastric cancer and has potential utility in HER2 testing of biopsy samples. J Clin Pathol. 2011;64(10):880–3.View ArticlePubMedGoogle Scholar
- Yang J, Luo H, Li Y, et al. Intratumoral heterogeneity determines discordant results of diagnostic tests for human epidermal growth factor receptor (HER) 2 in gastric cancer specimens. Cell Biochem Biophys. 2012;62(1):221–8.View ArticlePubMedGoogle Scholar
- Kim MA, Lee HJ, Yang HK, et al. Heterogeneous amplification of ERBB2 in primary lesions is responsible for the discordant ERBB2 status of primary and metastatic lesions in gastric carcinoma. Histopathology. 2011;59(5):822–31.PubMed CentralView ArticlePubMedGoogle Scholar
- Yoon HH, Shi Q, Sukov WR, et al. Adverse prognostic impact of intratumor heterogeneous HER2 gene amplification in patients with esophageal adenocarcinoma. J Clin Oncol. 2012;30(32):3932–8.PubMed CentralView ArticlePubMedGoogle Scholar
- Kimura Y, Oki E, Yoshida A, et al. Significance of accurate human epidermal growth factor receptor-2 (HER2) evaluation as a new biomarker in gastric cancer. Anticancer Res. 2014;34(8):4207–12.PubMedGoogle Scholar
- Bilous M, Osamura RY, Rüschoff J, et al. HER-2 amplification is highly homogenous in gastric cancer. Hum Pathol. 2010;41(2):304–5 (author reply 305-306).View ArticlePubMedGoogle Scholar
- NCCN guidelines gastric cancer, Principles of systemic therapy (GAST-E), 2012, < http://www.nccn.org/professionals/physician_gls/f_guidelines.asp#site>. Accessed 4 Aug 2016.
- Fassan M, Mastracci L, Grillo F, et al. Early HER2 dysregulation in gastric and oesophageal carcinogenesis. Histopathology. 2012;61:769–76.View ArticlePubMedGoogle Scholar
- Gómez-Martin C, Garralda E, Echarri MJ, et al. HER2/neu testing for anti-HER2-based therapies in patients with unresectable and/or metastatic gastric cancer. J Clin Pathol. 2012;65(8):751–7.PubMed CentralView ArticlePubMedGoogle Scholar
- Xu CC, Yue L, Wei HJ, et al. Significance of TFF3 protein and Her-2/neu status in patients with gastric adenocarcinoma. Pathol Res Pract. 2013;209(8):479–85.View ArticlePubMedGoogle Scholar
- Marx AH, Tharun L, Muth J, et al. HER-2 amplification is highly homogenous in gastric cancer. Human Pathol. 2009;40:769–77.View ArticleGoogle Scholar
- Kunz PL, Mojtahed A, Fisher GA, et al. HER2 expression in gastric and gastroesophageal junction adenocarcinoma in a US population: clinicopathologic analysis with proposed approach to HER2 assessment. Appl Immunohistochem Mol Morphol. 2012;20:13–24.View ArticlePubMedGoogle Scholar
- Kim MA, Jung EJ, Lee HS, et al. Evaluation of HER-2 gene status in gastric carcinoma using immunohistochemistry, fluorescence in situ hybridization, and real-time quantitative polymerase chain reaction. Human Pathol. 2007;38:1386–93.View ArticleGoogle Scholar
- Yan SY, HuY FJG, et al. Clinicopathologic significance of HER-2/neu protein expression and gene amplification in gastric carcinoma. World J Gastroenterol. 2011;17:1501–6.PubMed CentralView ArticlePubMedGoogle Scholar
- Moelans CB, Milne AN, Morsink FH, et al. Low frequency of HER2 amplification and overexpression in early onset gastric cancer. Cell Oncol (Dordrecht). 2011;34:89–95.View ArticleGoogle Scholar
- Fisher SB, Fisher KE, Squires 3rd MH, et al. HER2 in resected gastric cancer: is there prognostic value? J Surg Oncol. 2014;109(2):61–6.View ArticlePubMedGoogle Scholar
- Gomez-Martín C, Lopez-Rios F, Aparicio J, et al. A critical review of HER2-positive gastric cancer evaluation and treatment: from trastuzumab, and beyond. Cancer Lett. 2014;351(1):30–40.View ArticlePubMedGoogle Scholar
- Terashima M, Kitada K, Ochiai A, et al. Impact of expression of human epidermal growth factor receptors EGFR and ERBB2 on survival in stage II/III gastric cancer. Clin Cancer Res. 2012;18:5992–6000.View ArticlePubMedGoogle Scholar
- Aizawa M, Nagatsuma AK, Kitada K, et al. Evaluation of HER2-based biology in 1,006 cases of gastric cancer in a Japanese population. Gastric Cancer. 2014;17(1):34-42.Google Scholar
- Gordon MA, Gundacker HM, Benedetti J, et al. Assessment of HER2 gene amplification in adenocarcinomas of the stomach or gastroesophageal junction in the INT- 0116/SWOG9008 clinical trial. Ann Oncol. 2013;24(7):1754–61.PubMed CentralView ArticlePubMedGoogle Scholar
- Janjigian YY, Werner D, Pauligk C, et al. Prognosis of metastatic gastric and gastroesophageal junction cancer by HER2 status: a European and USA International collaborative analysis. Ann Oncol. 2012;23(10):2656–62.View ArticlePubMedGoogle Scholar
- Sukawa Y, Yamamoto H, Nosho K, et al. Alterations in the human epidermal growth factor receptor 2‐phosphatidylinositol 3‐kinase‐v‐Akt pathway in gastric cancer. World J Gastroenterol. 2012;18:6577–86.PubMed CentralView ArticlePubMedGoogle Scholar
- Tanner M, Hollmen M, Junttila TT, et al. Amplification of HER-2 in gastric carcinoma: association with topoisomerase II alpha gene amplification, intestinal type, poor prognosis and sensitivity to trastuzumab. Ann Oncol. 2005;16(2):273–8.View ArticlePubMedGoogle Scholar
- Bar-Sela G, Hershkovitz D, Haim N, et al. The incidence and prognostic value of HER2 overexpression and cyclin D1 expression in patients with gastric or gastroesophageal junction adenocarcinoma in Israel. Oncol Lett. 2013;5(2):559–63.PubMedGoogle Scholar
- Barros-Silva JD, Leitao D, Afonso L, et al. Association of ERBB2 gene status with histopathological parameters and disease-specific survival in gastric carcinoma patients. Br J Cancer. 2009;100(3):487–93.PubMed CentralView ArticlePubMedGoogle Scholar
- Park DI, Yun JW, Park JH, et al. HER-2/neu amplification is an independent prognostic factor in gastric cancer. Dig Dis Sci. 2006;51:1371–9.View ArticlePubMedGoogle Scholar
- Kataoka Y, Okabe H, Yoshizawa A, et al. HER2 expression and its clinicopathological features in resectable gastric cancer. Gastric Cancer. 2013;16:84–9.PubMedView ArticleGoogle Scholar
- Chua TC, Merret ND. Clinicopathologic factors associated with HER2-positive gastric cancer and its impact on survival outcomes—a systematic review. Int J Cancer. 2012;130:2845–56.View ArticlePubMedGoogle Scholar
- Power DG, Kelsen DP, Shah MA. Advanced gastric cancer—slow but steady progress. Cancer Treat Rev. 2010;36(5):384–92.View ArticlePubMedGoogle Scholar
- Boone JJM, Bhosle J, Tilby MJ, et al. Involvement of the HER2 pathway in repair of DNA damage produced by chemotherapeutic agents. Mol Cancer Ther. 2009;8:3015–23.View ArticlePubMedGoogle Scholar
- Satoh T, Xu RH, Chung HC, et al. Lapatinib plus paclitaxel versus paclitaxel alone in the second-line treatment of HER2-amplified advanced gastric cancer in Asian populations: TyTAN—a randomized, phase III study. J Clin Oncol. 2014;32:2039.PubMedView ArticleGoogle Scholar
- A study of trastuzumab emtansine versus taxane in patients with advanced gastric cancer https://clinicaltrials.gov/ct2/show/NCT01641939. Accessed 4 Aug 2016.
- Anbiaee R, Mojir Sheibani K, Torbati P, et al. Abnormal expression of e-cadherin in gastric adenocarcinoma, and its correlation with tumor histopathology and helicobacter pylori infection. Iran Red Crescent Med J. 2013;15(3):218–22.PubMedPubMed CentralView ArticleGoogle Scholar
- Carneiro P, Fernandes MS, Figueiredo J, et al. E-cadherin dysfunction in gastric cancer—cellular consequences, clinical applications and open questions. FEBS Lett. 2012;586:2981–9.View ArticlePubMedGoogle Scholar
- Christofori G, Semb H. The role of the cell-adhesion molecule E-cadherin as a tumour-suppressor gene. Trends Biochem Sci. 1999;24:73–6.View ArticlePubMedGoogle Scholar
- Chan AO. E-cadherin in gastric cancer. World J Gastroenterol. 2006;12:199–203.PubMed CentralView ArticlePubMedGoogle Scholar
- Tamura G. Alterations of tumor suppressor and tumor-related genes in the development and progression of gastric cancer. World J Gastroenterol. 2006;12:192–8.PubMed CentralView ArticlePubMedGoogle Scholar
- Corso G, Carvalho J, Marrelli D, et al. Somatic mutations and deletions of the E-cadherin gene predict poor survival of patients with gastric cancer. J Clin Oncol. 2013;31(7):868–75.View ArticlePubMedGoogle Scholar
- Ferreira P, Oliveira MJ, Beraldi E, et al. Loss of functional E-cadherin renders cells more resistant to the apoptotic agent taxol in vitro. Exp Cell Res. 2005;310(1):99–104.PubMedView ArticleGoogle Scholar
- Xin HW, Yang JH, Nguyen DM. Sensitivity to epidermal growth factor receptor tyrosine kinase inhibitor requires E-cadherin in esophageal cancer and malignant pleural mesothelioma. Anticancer Res. 2013;33(6):2401–8.PubMedGoogle Scholar
- Blair VR. Familial gastric cancer: genetics, diagnosis, and management. Surg Oncol Clin N Am. 2012;21:35–56.PubMedView ArticleGoogle Scholar
- Barber M, Murrell A, Ito Y, et al. Mechanisms and sequelae of E-cadherin silencing in hereditary diffuse gastric cancer. J Pathol. 2008;216:295–306.View ArticlePubMedGoogle Scholar
- Schrader K, Huntsman D. Hereditary diffuse gastric cancer. Cancer Treat Res. 2010;155:33–63.View ArticlePubMedGoogle Scholar
- Oliveira C, Senz J, Kaurah P, et al. Germline CDH1 deletions in hereditary diffuse gastric cancer families. Hum Mol Genet. 2009;18:1545–55.PubMed CentralView ArticlePubMedGoogle Scholar
- Pinheiro H, Bordeira-Carrico R, Seixas S, et al. Allele-specific CDH1 downregulation and hereditary diffuse gastric cancer. Hum Mol Genet. 2010;19:943–52.View ArticlePubMedGoogle Scholar
- Carneiro F, Huntsman DG, Smyrk TC, et al. Model of the early development of diffuse gastric cancer in E-cadherin mutation carriers and its implications for patient screening. J Pathol. 2004;203:681–7.View ArticlePubMedGoogle Scholar
- Knudson Jr AG. Mutation and cancer: statistical study of retinoblastoma. Proc Natl Acad Sci U S A. 1971;68:820–3.PubMedPubMed CentralView ArticleGoogle Scholar
- Knudson AG. Two genetic hits (more or less) to cancer. Nat Rev Cancer. 2001;1:157–62.PubMedView ArticleGoogle Scholar
- Oliveira C, de Bruin J, Nabais S, et al. Intragenic deletion of CDH1 as the inactivating mechanism of the wild-type allele in an HDGC tumour. Oncogene. 2004;23:2236–40.View ArticlePubMedGoogle Scholar
- Oliveira C, Sousa S, Pinheiro H, et al. Quantification of epigenetic and genetic 2nd hits in CDH1 during hereditary diffuse gastric cancer syndrome progression. Gastroenterology. 2009;136:2137–48.PubMedView ArticleGoogle Scholar
- Grady WM, Willis J, Guilford PJ, et al. Methylation of the CDH1 promoter as the second genetic hit in hereditary diffuse gastric cancer. Nat Genet. 2000;26:16–7.View ArticlePubMedGoogle Scholar
- Pharoah PD, Guilford P, Caldas C. Incidence of gastric cancer and breast cancer in CDH1 (E-cadherin) mutation carriers from hereditary diffuse gastric cancer families. Gastroenterology. 2001;121:1348–53.View ArticlePubMedGoogle Scholar
- Brooks-Wilson AR, Kaurah P, Suriano G, et al. Germline E-cadherin mutations in hereditary diffuse gastric cancer: assessment of 42 new families and review of genetic screening criteria. J Med Genet. 2004;41:508–17.PubMed CentralView ArticlePubMedGoogle Scholar
- Lynch HT, Grady W, Suriano G, et al. Gastric cancer: new genetic developments. J Surg Oncol. 2005;90:114–33 [discussion: 33].PubMedView ArticleGoogle Scholar
- Uppal DS, Powell SM. Genetics/genomics/proteomics of gastric adenocarcinoma. Gastroenterol Clin North Am. 2013;42(2):241–60.PubMedView ArticleGoogle Scholar
- Corso G, Pedrazzani C, Pinheiro H, et al. E-cadherin genetic screening and clinico-pathologic characteristics of early onset gastric cancer. Eur J Cancer. 2011;47:631–9.View ArticlePubMedGoogle Scholar
- Zhou YN, Xu CP, Han B, et al. Expression of E-cadherin and beta-catenin in gastric carcinoma and its correlation with the clinicopathological features and patient survival. World J Gastroenterol. 2002;8(6):987–93.PubMed CentralView ArticlePubMedGoogle Scholar
- Guzman P, Araya J, Villaseca M, et al. Immunohistochemical expression of the E-cadherin-catenin complex in gastric cancer. Rev Med Chil. 2006;134(8):1002–9.PubMedView ArticleGoogle Scholar
- Stone J, Bevan S, Cunningham D, et al. Low frequency of germline E-cadherin mutations in familial and nonfamilial gastric cancer. Br J Cancer. 1999;79:1935–7.PubMedPubMed CentralView ArticleGoogle Scholar
- Ascano JJ, Frierson Jr H, Moskaluk CA, et al. Inactivation of the E-cadherin gene in sporadic diffuse-type gastric cancer. Mod Pathol. 2001;14:942–9.View ArticlePubMedGoogle Scholar
- Graziano F, Arduini F, Ruzzo A, et al. Prognostic analysis of E-cadherin gene promoter hypermethylation in patients with surgically resected, node-positive, diffuse gastric cancer. Clin Cancer Res. 2004;10(8):2784–9.View ArticlePubMedGoogle Scholar
- Jawhari A, Jordan S, Poole S, et al. Abnormal immunoreactivity of the E-cadherin-catenin complex in gastric carcinoma: relationship with patient survival. Gastroenterology. 1997;112(1):46–54.View ArticlePubMedGoogle Scholar
- Gamboa-Dominguez A, Dominguez-Fonseca C, Chavarri-Guerra Y, et al. E-cadherin expression in sporadic gastric cancer from Mexico: exon 8 and 9 deletions are infrequent events associated with poor survival. Hum Pathol. 2005;36:29–35.View ArticlePubMedGoogle Scholar
- Deng N, Goh LK, Wang H, et al. A comprehensive survey of genomic alterations in gastric cancer reveals systematic patterns of molecular exclusivity and co-occurrence among distinct therapeutic targets. Gut. 2012;61:673–84.PubMedPubMed CentralView ArticleGoogle Scholar
- Singh SS, Yap WN, Arfuso F, et al. Targeting the PI3K/Akt signaling pathway in gastric carcinoma: a reality for personalized medicine? World J Gastroenterol. 2015;21(43):12261–73.PubMedPubMed CentralView ArticleGoogle Scholar
- Velho S, Oliveira C, Ferreira A, et al. The prevalence of PIK3CA mutations in gastric and colon cancer. Eur J Cancer. 2005;41(11):1649–54.PubMedView ArticleGoogle Scholar
- Lee J, van Hummelen P, Go C, et al. High-throughput mutation profiling identifies frequent somatic mutations in advanced gastric adenocarcinoma. PLoS One. 2012;7(6):e38892.PubMed CentralView ArticlePubMedGoogle Scholar
- Shi J, Yao D, Liu W, et al. Highly frequent PIK3CA amplification is associated with poor prognosis in gastric cancer. BMC Cancer. 2012;12:50.PubMedPubMed CentralView ArticleGoogle Scholar
- Barbi S, Cataldo I, De Manzoni G, et al. The analysis of PIK3CA mutations in gastric carcinoma and metanalysis of literature suggest that exon- selectivity is a signature of cancer type. J Exp Clin Cancer Res. 2010;29:32.PubMedPubMed CentralView ArticleGoogle Scholar
- Xu DZ, Geng QR, Tian Y, et al. Activated mammalian target of rapamycin is a potential therapeutic target in gastric cancer. BMC Cancer. 2010;10:536.PubMedPubMed CentralView ArticleGoogle Scholar
- Yu G, Wang J, Chen Y, et al. Overexpression of phosphorylated mammalian target of rapamycin predicts lymph node metastasis and prognosis of Chinese patients with gastric cancer. Clin Cancer Res. 2009;15(5):1821–9.PubMedView ArticleGoogle Scholar
- Doi T, Muro K, Boku N, et al. Multicenter phase II study of everolimus in patients with previously treated metastatic gastric cancer. J Clin Oncol. 2010;28:1904.PubMedView ArticleGoogle Scholar
- Yoon DH, Ryu MH, Park YS, et al. Phase II study of everolimus with biomarker exploration in patients with advanced gastric cancer refractory to chemotherapy including fluoropyrimidine and platinum. Br J Cancer. 2012;106:1039.PubMedPubMed CentralView ArticleGoogle Scholar
- Ohtsu A, Ajani JA, Bai YX, et al. Everolimus for previously treated advanced gastric cancer: results of the randomized, double-blind, phase III GRANITE-1 study. J Clin Oncol. 2013;31:3935.PubMedView ArticleGoogle Scholar
- Kim MA, Lee HS, Lee HE, et al. EGFR in gastric carcinomas, prognostic significance of protein over-expression and high gene copy number. Histopathology. 2008;52(6):738–46.PubMedView ArticleGoogle Scholar
- Kiyose S, Nagura K, Tao H, et al. Detection of kinase amplifications in gastric cancer archives using fluorescence in situ hybridization. Pathol Int. 2012;62(7):477–84.PubMedView ArticleGoogle Scholar
- Lordick F, Kang YK, Chung HC, et al. Capecitabine and cisplatin with or without cetuximab for patients with previously untreated advanced gastric cancer (EXPAND): a randomized, open-label phase 3 trial. Lancet Oncol. 2013;14:490.View ArticlePubMedGoogle Scholar
- Waddell T, Chau I, Cunningham D, et al. Epirubicin, oxaliplatin, and capecitabine with or without panitumumab for patients with previously untreated advanced oesophagogastric cancer (REAL3): a randomised, open-label phase 3 trial. Lancet Oncol. 2013;14:481.PubMedPubMed CentralView ArticleGoogle Scholar
- Dragovich T, McCoy S, Fenoglio-Preiser C, et al. Phase II trial of erlotinib in gastroesophageal junction and gastric adenocarcinomas: SWOG 0127. J Clin Oncol. 2006;24:4922–7.View ArticlePubMedGoogle Scholar
- Pietrantonio F, De Braud F, Da Prat V, et al. A review on biomarkers for prediction of treatment outcome in gastric cancer. Anticancer Res. 2013;33(4):1257–66.PubMedGoogle Scholar
- Graziano F, Galluccio N, Lorenzini P, et al. Genetic activation of the MET pathway and prognosis of patients with high-risk, radically resected gastric cancer. J Clin Oncol. 2011;29(36):4789–95.PubMedView ArticleGoogle Scholar
- Lee J, Seo JW, Jun HJ, et al. Impact of MET amplification on gastric cancer: possible roles as a novel prognostic marker and a potential therapeutic target. Oncol Rep. 2011;25(6):1517–24.PubMedGoogle Scholar
- Lennerz JK, Kwak EL, Ackerman A, et al. MET amplification identifies a small and aggressive subgroup of esophagogastric adenocarcinoma with evidence of responsiveness to crizotinib. J Clin Oncol. 2011;29(36):4803–10.PubMedPubMed CentralView ArticleGoogle Scholar
- Lee HE, Kim MA, Lee HS, et al. MET in gastric carcinomas: comparison between protein expression and gene copy number and impact on clinical outcome. Br J Cancer. 2012;107(2):325–33.PubMedPubMed CentralView ArticleGoogle Scholar
- Janjigian YY, Tang LH, Coit DG, et al. MET expression and amplification in patients with localized gastric cancer. Cancer Epidemiol Biomarkers Prev. 2011;20(5):1021–7.PubMedPubMed CentralView ArticleGoogle Scholar
- Sehdev A, Catenacci DV. Gastroesophageal cancer: focus on epidemiology, classification, and staging. Discov Med. 2013;16(87):103–11.PubMedGoogle Scholar
- Shah MA, Cho JY, Huat IT, et al. Randomized phase II study of FOLFOX +/- MET inhibitor, onartuzumab (O), in advanced gastroesophageal adenocarcinoma (GEC). J Clin Oncol. 2015;33:suppl 3; abstr 2.View ArticleGoogle Scholar
- Jhawer M, Kindler H, Wainberg Z, et al. Assessment of two dosing schedules of GSK1363089 (GSK089), a dual MET/VEGFR2 inhibitor, in metastatic gastric cancer (GC): interim results of a multicenter phase II study. J Clin Oncol. 2009;27(15 Suppl):abstract 4502.Google Scholar
- Fife BT, Pauken KE. The role of the PD-1 pathway in autoimmunity and peripheral tolerance. Ann N Y Acad Sci. 2011;1217:45–59.PubMedView ArticleGoogle Scholar
- Raufi AG, Klempner SJ. Immunotherapy for advanced gastric and esophageal cancer: preclinical rationale and ongoing clinical investigations. J Gastrointest Oncol. 2015;6(5):561–9.PubMed CentralPubMedGoogle Scholar
- Muro K, Bang Y-J, Shankaran V, et al. Relationship between PD-L1 expression and clinical outcomes in patients with advanced gastric cancer treated with the anti-PD-1 monoclonal antibody pembrolizumab in KEYNOTE-012 (abstract). J Clin Oncol. 2015;33:suppl 3; abstr 3.Google Scholar
- Belyi VA, Ak P, Markert E, et al. The origins and evolution of the p53 family of genes. Cold Spring Harbor Perspectives in Biology. 2010;2(6):a001198.PubMed CentralView ArticlePubMedGoogle Scholar
- Bellini MF, Cadamuro AC, Succi M, et al. Alterations of the TP53 gene in gastric and esophageal carcinogenesis. J Biomed Biotechnol. 2012;2012:891961.PubMedPubMed CentralView ArticleGoogle Scholar
- Oki E, Zhao Y, Yoshida R, et al. The difference in p53 mutations between cancers of the upper and lower gastrointestinal tract. Digestion. 2009;79(1):33–9.PubMedView ArticleGoogle Scholar
- Iwamatsu H, Nishikura K, Watanabe H, et al. Heterogeneity of p53 mutational status in the superficial spreading type of early gastric carcinoma. Gastric Cancer. 2001;4(1):20–6.PubMedView ArticleGoogle Scholar
- Liu XP, Tsushimi K, Tsushimi M, et al. Expression of p53 protein as a prognostic indicator of reduced survival time in diffuse-type gastric carcinoma. Pathol Int. 2001;51(6):440–4.PubMedView ArticleGoogle Scholar
- Fenoglio-Preiser CM, Wang J, Stemmermann GN, et al. TP53 and gastric carcinoma: a review. Human Mutation. 2003;21(3):258–70.PubMedView ArticleGoogle Scholar
- Lu HZ, Wu YP, Luo W, et al. Correlation between aneuploidy of chromosome 17, over-expression of TP53 and TOP-II alpha, and the clinicopathological features and diagnosis of gastric adenocarcinoma. Zhonghua Zhong Liu Za Zhi. 2009;31(10):754–8.PubMedGoogle Scholar
- Corso G, Pedrazzani C, Marelli D, et al. Correlation of microsatellite instability at multiple loci with long term survival in advanced gastric carcinoma. Arch Surg. 2009;144:722–7.PubMedView ArticleGoogle Scholar
- Leung WK, Kim JJ, Kim JG, et al. Microsatellite instability in gastric intestinal metaplasia in patients with and without gastric cancer. Am J Pathol. 2000;156:537–43.PubMedPubMed CentralView ArticleGoogle Scholar
- Pinto M, Oliveira C, Machado JC, et al. MSI-L gastric carcinomas share the hMLH1 methylation status of MSI-H carcinomas but not their clinicopathological profile. Lab Invest. 2000;80(12):1915–23.View ArticlePubMedGoogle Scholar
- Velho S, Fernandes MS, Leite M, et al. Causes and consequences of microsatellite instability in gastric carcinogenesis. World J Gastroenterol. 2014;20(44):16433–42.PubMed CentralView ArticlePubMedGoogle Scholar
- Corso G, Velho S, Paredes J, et al. Oncogenic mutations in gastric cancer with microsatellite instability. Eur J Cancer. 2011;47(3):443–51.View ArticlePubMedGoogle Scholar
- Oliveira C, Seruca R, Seixas M, et al. The clinicopathological features of gastric carcinomas with microsatellite instability may be mediated by mutations of different “target genes”: a study of the TGFbeta RII, IGFII R, and BAX genes. Am J Pathol. 1998;153(4):1211–9.PubMed CentralView ArticlePubMedGoogle Scholar
- Nakajima T, Akiyama Y, Shiraishi J, et al. Age-related hypermethylation of the hMLH1 promoter in gastric cancers. Int J Cancer. 2001;94:208–11.View ArticlePubMedGoogle Scholar
- Pedrazzani C, Corso G, Velho S, et al. Evidence of tumor microsatellite instability in gastric cancer with familial aggregation. Fam Cancer. 2009;8:215–20.View ArticlePubMedGoogle Scholar
- dos Santos NR, Seruca R, Constância M, et al. Microsatellite instability at multiple loci in gastric carcinoma: clinicopathologic implications and prognosis. Gastroenterology. 1996;110:38–44.View ArticlePubMedGoogle Scholar
- Seruca R, Santos NR, David L, et al. Sporadic gastric carcinomas with microsatellite instability display a particular clinicopathologic profile. Int J Cancer. 1995;64:32–6.View ArticlePubMedGoogle Scholar
- Beghelli S, de Manzoni G, Barbi S, et al. Microsatellite instability in gastric cancer is associated with better prognosis in only stage II cancers. Surgery. 2006;139:347–56.View ArticlePubMedGoogle Scholar
- Han TS, Hur K, Xu G, et al. MicroRNA-29c mediates initiation of gastric carcinogenesis by directly targeting ITGB1. Gut. 2015;64(2):203–14.View ArticlePubMedGoogle Scholar
- Su ZX, Zhao J, Rong ZH, et al. Diagnostic and prognostic value of circulating miR-18a in the plasma of patients with gastric cancer. Tumour Biol. 2014;35(12):12119–25.View ArticlePubMedGoogle Scholar
- Zhu X, Lv M, Wang H, et al. Identification of circulating microRNAs as novel potential biomarkers for gastric cancer detection: a systematic review and meta-analysis. Dig Dis Sci. 2014;59(5):911–9.View ArticlePubMedGoogle Scholar
- Zhu Z, Zhang X, Wang G, et al. Role of MICRORNAS in hepatocellular carcinoma. Hepat Mon. 2014;14:e18672.PubMed CentralView ArticlePubMedGoogle Scholar
- Huang D, Wang H, Liu R, et al. miRNA27a is a biomarker for predicting chemosensitivity and prognosis in metastatic or recurrent gastric cancer. J Cell Biochem. 2014;115(3):549–56.View ArticlePubMedGoogle Scholar
- Ma GJ, Gu RM, Zhu M, et al. Plasma post-operative miR-21 expression in the prognosis of gastric cancers. Asian Pac J Cancer Prev. 2013;14(12):7551–4.View ArticlePubMedGoogle Scholar
- Gilad S, Meiri E, Yogev Y, et al. Serum microRNAs are promising novel biomarkers. PLoS ONE. 2008;3(9):e3148.PubMed CentralView ArticlePubMedGoogle Scholar
- Etheridge A, Lee I, Hood L, et al. Extracellular microRNA: a new source of biomarkers. Mutat Res. 2011;717(1–2):85–90.PubMed CentralView ArticlePubMedGoogle Scholar
- Vosa U, Vooder T, Kolde R, et al. Meta-analysis of microRNA expression in lung cancer. Int J Cancer. 2013;132:2884–93.View ArticlePubMedGoogle Scholar
- Gong X, Wu R, Wang H, et al. Evaluating the consistency of differential expression of microRNA detected in human cancers. Mol Cancer Ther. 2011;10:752–60.View ArticlePubMedGoogle Scholar
- Shrestha S, Hsu SD, Huang WY, et al. A systematic review of microRNA expression profiling studies in human gastric cancer. Cancer Med. 2014;3(4):878–88.PubMed CentralView ArticlePubMedGoogle Scholar
- Liu HS, Xiao HS. MicroRNAs as potential biomarkers for gastric cancer. World J Gastroenterol. 2014;20(34):12007–17.PubMed CentralView ArticlePubMedGoogle Scholar
- Wu HH, Lin WC, Tsai KW. Advances in molecular biomarkers for gastric cancer: miRNAs as emerging novel cancer markers. Expert Rev Mol Med. 2014;16:e1.PubMed CentralView ArticlePubMedGoogle Scholar
- Fang XY, Pan HF, Leng RX, et al. Long noncoding RNAs: novel insights into gastric cancer. Cancer Lett. 2015;356(2 Pt B):357–66.View ArticlePubMedGoogle Scholar
- Kaikkonen MU, Lam MT, Glass CK. Non-coding RNAs as regulators of gene expression and epigenetics. Cardiovasc Res. 2011;90:430–40.PubMed CentralView ArticlePubMedGoogle Scholar
- Yang L, Froberg JE, Lee JT. Long noncoding RNAs: fresh perspectives into the RNA world. Trends Biochem Sci. 2014;39:35–43.View ArticlePubMedGoogle Scholar
- Kornienko AE, Guenzl PM, Barlow DP, et al. Gene regulation by the act of long non-coding RNA transcription. BMC Biol. 2013;11:59.PubMed CentralView ArticlePubMedGoogle Scholar
- Salmena L, Poliseno L, Tay Y, et al. A ceRNA hypothesis: the Rosetta Stone of a hidden RNA language? Cell. 2011;146:353–8.PubMed CentralView ArticlePubMedGoogle Scholar
- Song H, Sun W, Ye G, et al. Long non-coding RNA expression profile in human gastric cancer and its clinical significances. J Transl Med. 2013;11:225.PubMed CentralView ArticlePubMedGoogle Scholar
- Sampieri CL, León-Córdoba K, Remes-Troche JM. Matrix metalloproteinases and their tissue inhibitors in gastric cancer as molecular markers. J Cancer Res Ther. 2013;9(3):356–63.View ArticlePubMedGoogle Scholar
- Baker AH, Edwards DR, Murphy G. Metalloproteinase inhibitors: biological actions and therapeutic opportunities. J Cell Sci. 2002;115:3719–27.View ArticlePubMedGoogle Scholar
- Swarnakar S, Paul S, Singh LP, et al. Matrix metalloproteinases in health and disease: regulation by melatonin. J Pineal Res. 2011;50(1):8–20.View ArticlePubMedGoogle Scholar
- Koskensalo S, Mrena J, Wiksten JP, et al. MMP-7 overexpression is an independent prognostic marker in gastric cancer. Tumour Biol. 2010;31(3):149–55.View ArticlePubMedGoogle Scholar
- Shi M, Liu D, Duan H, et al. Catecholamine up-regulates MMP-7 expression by activating AP-1 and STAT3 in gastric cancer. Mol Cancer. 2010;9:269.PubMed CentralView ArticlePubMedGoogle Scholar
- Al-Batran SE, Pauligk C, Wirtz R, et al. The validation of matrix metalloproteinase-9 mRNA gene expression as a predictor of outcome in patients with metastatic gastric cancer. Ann Oncol. 2012;23(7):1699–705.View ArticlePubMedGoogle Scholar
- Chang WJ, Du Y, Zhao X, et al. Inflammation-related factors predicting prognosis of gastric cancer. World J Gastroenterol. 2014;20(16):4586–96.PubMed CentralView ArticlePubMedGoogle Scholar
- Long ZW, Wang JL, Wang YN. Matrix metalloproteinase-7 mRNA and protein expression in gastric carcinoma: a meta-analysis. Tumour Biol. 2014;35(11):11415–26.View ArticlePubMedGoogle Scholar
- Yonemura Y, Fujimura T, Ninomiya I, et al. Prediction of peritoneal micrometastasis by peritoneal lavaged cytology and reverse transcriptase-polymerase chain reaction for matrix metalloproteinase-7 mRNA. Clin Cancer Res. 2001;7:1647–53.PubMedGoogle Scholar
- Wu CY, Wu MS, Chiang EP, et al. Plasma matrix metalloproteinase-9 level is better than serum matrix metalloproteinase-9 level to predict gastric cancer evolution. Clin Cancer Res. 2007;13:2054–60.View ArticlePubMedGoogle Scholar
- Chu D, Zhang Z, Li Y, et al. Matrix metalloproteinase-9 is associated with disease-free survival and overall survival in patients with gastric cancer. Int J Cancer. 2011;129(4):887–95.View ArticlePubMedGoogle Scholar
- Wu T, Li Y, Lu J, et al. Increased MMP-21 expression is associated with poor overall survival of patients with gastric cancer. Med Oncol. 2013;30(1):323.View ArticlePubMedGoogle Scholar
- Wu KP, Li Q, Lin FX, et al. MT1-MMP is not a good prognosticator of cancer survival: evidence from 11 studies. Tumour Biol. 2014;35(12):12489–95.View ArticlePubMedGoogle Scholar
- Peng CW, Wang LW, Fang M, et al. Combined features based on MT1-MMP expression, CD11b + immunocytes density and LNR predict clinical outcomes of gastric cancer. J Transl Med. 2013;11:153.PubMed CentralView ArticlePubMedGoogle Scholar
- Shim KN, Jung SA, Joo YH, et al. Clinical significance of tissue levels of matrix metalloproteinases and tissue inhibitors of metalloproteinases in gastric cancer. J Gastroenterol. 2007;42:120–8.View ArticlePubMedGoogle Scholar
- He L, Chu D, Li X, et al. Matrix metalloproteinase-14 is a negative prognostic marker for patients with gastric cancer. Dig Dis Sci. 2013;58:1264–70.View ArticlePubMedGoogle Scholar
- Bramhall S, Hallissey M, Whiting J, et al. Marimastat as maintenance therapy for patients with advanced gastric cancer: a randomised trial. Br J Cancer. 2002;86:1864–70.PubMed CentralView ArticlePubMedGoogle Scholar
- Rugge M, Capelle LG, Fassan M. Individual risk stratification of gastric cancer: evolving concepts and their impact on clinical practice. Best Pract Res Clin Gastroenterol. 2014;28(6):1043–53.View ArticlePubMedGoogle Scholar
- Riquelme I, Saavedra K, Espinoza JA, et al. Molecular classification of gastric cancer: towards a pathway-driven targeted therapy. Oncotarget. 2015;6(28):24750–79.PubMed CentralView ArticlePubMedGoogle Scholar
- Wadhwa R, Song S, Lee JS, et al. Gastric cancer-molecular and clinical dimensions. Nat Rev Clin Oncol. 2013;10(11):643–55.PubMed CentralView ArticlePubMedGoogle Scholar
- Ali SM, Sanford EM, Klempner SJ. Prospective comprehensive genomic profiling of advanced gastric carcinoma cases reveals frequent clinically relevant genomic alterations and new routes for targeted therapies. Oncologist. 2015;20(5):499–507.PubMed CentralView ArticlePubMedGoogle Scholar