- Open Access
Inflammation-based prognostic system predicts postoperative survival of esophageal carcinoma patients with normal preoperative serum carcinoembryonic antigen and squamous cell carcinoma antigen levels
- Qilong Ma†1,
- Wengao Liu†1,
- Ran Jia1,
- Feng Jiang1,
- Hao Duan1,
- Peng Lin1, 2,
- Lanjun Zhang1, 2,
- Hao Long1,
- Hongyun Zhao1, 4Email author and
- Guowei Ma1, 3Email author
© Ma et al. 2016
- Received: 9 June 2015
- Accepted: 19 April 2016
- Published: 5 May 2016
The Glasgow Prognostic Score (GPS) is an established inflammation-based system that is used to predict the prognosis for several types of malignancies. In this retrospective study, we assessed the postoperative survival of 725 patients with non-metastatic esophageal squamous cell carcinoma who had normal preoperative serum tumor marker levels according to the GPS.
Among 1394 patients who underwent esophagectomy between August 2006 and December 2010, 725 with normal preoperative serum levels of carcinoembryonic antigen (CEA) and squamous cell carcinoma antigen (SCC-Ag) were enrolled. All demographic, pathologic, and survival data were analyzed retrospectively. Uni- and multivariate analyses were performed to evaluate the relationship with overall survival. The Kaplan–Meier analysis and log-rank tests were used to compare the survival curves between patients with GPS 0 (group A) and 1 or 2 (group B).
Patients in group A exhibited significantly better 3- and 5-year cancer-specific survival (CSS) rates (0.780 and 0.759, respectively) than those in group B (0.624 and 0.605, respectively). Multivariate Cox regression analysis revealed that age, tumor length, pathological tumor-node-metastasis (pTNM) stage, venous invasion, lymph node metastasis, serum albumin and C-reactive protein levels, and GPS were associated with postoperative survival of these patients. Further multivariate analysis confirmed that GPS was an independent prognostic factor. The Kaplan–Meier analysis and log-rank tests demonstrated a significant difference in CSS between groups A and B (P = 0.001).
GPS may be a valuable prognostic indicator for esophageal cancer patients with normal preoperative CEA and SCC-Ag serum levels.
- Esophageal carcinoma
- Carcinoembryonic antigen
- Squamous cell carcinoma antigen
- Postoperative survival
- Glasgow Prognostic Score
Esophageal cancer is one of the most common malignancies worldwide, ranking sixth in terms of cancer-related mortality . It is prevalent in China, Iran, South Africa, Uruguay, France, and Italy. However, almost half of new esophageal cancer cases occur in China, resulting in the highest mortality rate . Importantly, squamous cell carcinoma, which accounts for >95 % of esophageal cancer cases, is the major histological subtype in China . Despite improvements in less invasive treatment strategies, surgery remains the mainstay of curative management. Unfortunately, the outcome of surgical resection for esophageal cancer remains poor with a postoperative 5-year survival rate of only 20–40 % in China .
Multiple tumor markers, such as carcinoembryonic antigen (CEA) and squamous cell carcinoma antigen (SCC-Ag), are widely used in clinical practice to estimate the prognosis of patients with esophageal cancer. At our institution, serum levels of CEA and SCC-Ag are routinely measured in patients with esophageal cancer prior to treatment. However, even patients with metastatic disease may not have elevated serum levels of CEA or SCC-Ag before or after surgery [5–7]. Therefore, these tumor makers cannot be applied widely for the prediction of postoperative survival. On the other hand, inflammation-based prognosis using indicators such as the Glasgow Prognostic Score (GPS) has been shown to be a valuable predictor of survival after surgery [8–10]. Because the GPS is thought to reflect the systemic inflammatory response (SIR) on the basis of hypercytokinemia originating from the interaction between the tumor and the host, there may be significant differences between the prognoses made using GPS and tumor markers . Therefore, we hypothesized that GPS is a useful prognostic indicator of postoperative survival in patients with esophageal cancer, especially in those who have normal preoperative serum CEA and SCC-Ag levels. We tested this hypothesis in a retrospective study of 725 patients who had undergone esophagectomy due to esophageal cancer who had normal preoperative serum CEA and SCC-Ag levels.
Among 1394 patients who underwent esophagectomy at the Department of Thoracic Surgery of Sun Yat-sen University Cancer Center (Guangzhou, China) between August 2006 and December 2010, a total of 725 patients with esophageal cancer were retrospectively enrolled in the present study. Patients eligible for this cohort study had pathologically confirmed esophageal squamous cell carcinoma (ESCC). In all patients, the preoperative serum levels of CEA and SCC-Ag were ≤5.0 ng/ml and ≤1.5 μg/l, respectively. Each patient underwent esophagectomy. Routine laboratory testing of serum levels of C-reactive protein (CRP), albumin (ALB), and tumor markers, including CEA and SCC-Ag, was performed on the day of admission to exclude any effect associated with interference from successive preoperative examinations [12–15]. Patients were excluded if they had previously received cytotoxic chemotherapy or radiotherapy or had a past or current history of another malignancy. Patients were not eligible if tumors were located at the cervical esophagus or esophagogastric junction or had other histological subtypes of esophageal cancer besides ESCC. None of the patients exhibited clinical evidence of infection or other inflammatory conditions, and none received preoperative chemotherapy or irradiation. All patients were staged according to the 7th Edition of the American Joint Committee on Cancer (AJCC) Cancer Staging Manual. The study protocol was approved by the Ethics Committee of Sun Yat-sen University Cancer Center.
The standard surgical approaches consisted of the Sweet (left thoracotomy and diaphragm incision), the McKeown (right thoracotomy, laparotomy, and neck incision), and the Ivor Lewis (laparotomy and right thoracotomy) procedures. In our institute, the majority of patients underwent the Sweet surgical procedure. In this cohort of patients, thoracoabdominal lymphadenectomy was performed.
Patients were recommended for follow-up examinations at our outpatient department every 3 months for the first 2 years, every 6 months during the subsequent 3 years, and annually thereafter. Follow-up examinations consisted of history taking, physical examination, barium esophagography, chest radiography, abdominal ultrasonography, cervical ultrasonography, and neck-abdomen CT scans. Patients underwent endoscopy and/or positron emission tomography-CT, if necessary.
GPS was estimated as described previously. In brief, patients with both elevated CRP levels (>1.0 mg/dl) and hypoalbuminemia (<3.5 g/dl) were allocated a GPS of 2. Patients with only one of these biochemical abnormalities were allocated a GPS of 1, and those with neither abnormality were allocated a GPS of 0.
For analysis, each patient was allocated to one of two groups: group A (GPS = 0) or group B (GPS = 1 or 2). Patients with a GPS of 1 or 2 were combined because only nine patients had a GPS of 2.
Statistical analysis was performed using SPSS Statistics 18.0 software (IBM SPSS, Inc., Chicago, IL, USA). Odds ratios (OR) with 95 % confidence intervals (95 % CIs) were calculated by multivariate logistic regression analysis. Multivariate analysis was performed to evaluate the influences of sex, age, maximum tumor diameter, smoking history, history of alcohol consumption, intraoperative blood loss, venous invasion, lymph node metastasis, serum CRP, CEA and SCC-Ag levels, pathological tumor-node-metastasis (pTNM) stage, and GPS on cancer-specific survival (CSS). A two-sided P < 0.050 was considered statistically significant.
The most valuable prognostic factors identified by univariate analysis were confirmed by multivariate analysis. Multivariate Cox regression analysis was used to exclude other confounding factors affecting survival. The selected variable with univariate analysis was P < 0.05. Similarly, Kaplan–Meier analysis and the log-rank tests were used to compare survival curves between groups. Cases were censored at death or the end of follow-up.
Baseline characteristics of patients grouped according to the Glasgow Prognostic Score
(n = 616, 85 %)
(n = 109, 15 %)
(χ 2 test)
564 (77.79 %)
92 (12.69 %)
52 (7.17 %)
17 (2.34 %)
452 (62.34 %)
87 (12.00 %)
164 (22.62 %)
22 (3.03 %)
377 (52.00 %)
75 (10.34 %)
239 (32.97 %)
34 (4.69 %)
History of alcohol consumption
205 (28.28 %)
45 (6.21 %)
411 (56.69 %)
63 (8.69 %)
Tumor length (cm)
474 (65.38 %)
52 (7.17 %)
132 (18.21 %)
57 (7.86 %)
Intraoperative blood loss (ml)
471 (64.97 %)
77 (10.62 %)
143 (19.72 %)
31 (4.28 %)
92 (12.69 %)
11 (1.52 %)
299 (41.24 %)
46 (6.34 %)
225 (31.03 %)
52 (7.17 %)
603 (83.17 %)
107 (14.76 %)
13 (1.79 %)
2 (0.28 %)
Lymph node metastasis
332 (45.79 %)
56 (7.72 %)
284 (39.17 %)
53 (7.31 %)
Univariate analysis of factors related to esophageal cancer survival
95 % CI for Exp(B)
Age (≤65 or >65 years)
Sex (male or female)
History of alcohol consumption (ml)
Tumor length (cm)
Intraoperative blood loss (ml)
Lymph node metastasis
Multivariate analysis of selected clinical characteristics in relation to overall survival
95 % CI for Exp(B)
Tumor length (cm)
Lymph node metastasis
The prognosis for esophageal carcinoma patients is influenced by various pathological characteristics. The tumor markers CEA, SCC-Ag, CA19-9, and CYFRA 21-1 are commonly used to estimate the preoperative tumor status as well as postoperative survival and recurrence in patients with esophageal cancer [5, 6, 16]. Before 2010, the serum levels of CEA and SCC-Ag were measured routinely prior to treatment in all patients at our institution. Therefore, CEA and SCC-Ag were adopted as the two prognostic markers in the present study. However, many patients with metastatic disease may not have elevated CEA or SCC-Ag serum levels before or after surgery [5–7]. This study was undertaken to investigate whether GPS, measured prior to treatment, is useful in predicting the postoperative survival of patients with esophageal cancer who have normal preoperative serum CEA and SCC-Ag levels. Of interest, multivariate analyses revealed that GPS was associated with postoperative survival, indicating that GPS, measured at admission prior to treatment, is a useful predictor of postoperative outcome.
Recently, several reports have indicated that the presence of a systemic inflammatory response is a useful indicator of outcome among patients with esophageal cancer [17–21]. However, few studies have investigated whether GPS is useful for predicting postoperative outcome in ESCC patients with normal preoperative serum CEA and SCC-Ag levels.
The causal relationship between GPS and cancer survival may be due to the presence of a systemic inflammatory response and the influence of the associated nutritional decline [22, 23]. GPS may reflect hypercytokinemia resulting from immune cell activation as part of tumor versus host response, which may differ from the mechanism of tumor progression indicated by tumor marker levels [24, 25]. Due to the presence of cachexia caused by SIR-induced hypercytokinemia, patients in group B were expected to exhibit fewer clinical characteristics on the basis of nutritional status, neutrophil/lymphocyte ratio, and serum ALB level than those in group A.
As mentioned previously, GPS appears to be a significant prognostic factor that can be used to facilitate an accurate estimation of tumor characteristics in select populations. The results of our study showed that CSS of group A was superior to that of group B. Interestingly, univariate analysis identified two types of clinical characteristics as significant prognostic factors for CSS in patients with esophageal cancer: tumor-related characteristics, such as lymph node metastasis, tumor length, venous invasion, lymph node metastasis and pTNM stage (I, II, and III), and SIR-related characteristics, such as the serum levels of ALB and CRP and the GPS (0/1, 2) . It is well-known that the latter three indicators are associated with hypercytokinemia. Moreover, among the clinical characteristics identified in the univariate analysis, multivariate analysis clearly demonstrated that the inflammation-based prognostic indicator GPS was associated with CSS. Although there were three SIR-related characteristics which were associated with CSS, Kaplan–Meier analysis revealed that patients could be divided into two independent groups on the basis of GPS (GPS 0/1, 2).
The levels of CRP, albumin, CEA, SCC-Ag, and other serum indexes are routinely measured in patients. Consequently, it is simple to evaluate the GPS for each patient prior to treatment. Thus, the pretreatment GPS is an easily measurable and valuable preoperative prognostic indicator for esophageal cancer patients with normal preoperative CEA and SCC-Ag serum levels.
This work was supported by the Science and Technology Planning Project of Guangdong Province, China (No. 2012B031800463), and the Science and Technology Planning Project of Guangdong Province, China (No. 2013B022000040). The authors thank Enago (www.enago.cn) for the English language review.
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- Jemal A, Siegel R, Xu JQ, Ward E. Cancer statistics, 2010. CA Cancer J Clin. 2010;60:277–300.View ArticlePubMedGoogle Scholar
- Lu S, Lin P, Wang G, Luo X, Wu M. Comprehensive prevention and treatment for esophageal cancer. Chin Med J. 1999;112:918–23.PubMedGoogle Scholar
- Mao YS, He J, Cheng GY. Current status of surgical management of esophageal cancer in China and the future strategy. Zhonghua Zhong Liu Za Zhi. 2010;32(6):401–4.PubMedGoogle Scholar
- Liu JF, Wang QZ, Hou J. Surgical treatment for cancer of the oesophagus and gastric cardia in Hebei, China. Br J Surg. 2004;91:90–8.View ArticlePubMedGoogle Scholar
- Kosugi S, Nishimaki T, Kanda T, et al. Clinical significance of serum carcinoembryonic antigen, carbohydrate antigen 19-9, and squamous cell carcinoma antigen levels in esophageal cancer patients. World J Surg. 2004;28(7):680–5.View ArticlePubMedGoogle Scholar
- Mroczko B, Kozlowski M, Groblewska M, et al. The diagnostic value of the measurement of matrix metalloproteinase 9 (MMP-9), squamous cell cancer antigen (SCC) and carcinoembryonic antigen (CEA) in the sera of esophageal cancer patients. Clin Chim Acta. 2008;389(1–2):61–6.View ArticlePubMedGoogle Scholar
- Suminami Y, Nawata S, Kato H. Biological role of SCC antigen. Tumour Biol. 1998;19(6):488–93.View ArticlePubMedGoogle Scholar
- Ishizuka M, Nagata H, Takagi K, Horie T, Kubota K. Inflammation based prognostic score is a novel predictor of postoperative outcome in patients with colorectal cancer. Ann Surg. 2007;246:1047–51.View ArticlePubMedGoogle Scholar
- Ramsey S, Lamb GW, Aitchison M, Graham J, McMillan DC. Evaluation of an inflammation-based prognostic score in patients with metastatic renal cancer. Cancer. 2007;109:205–12.View ArticlePubMedGoogle Scholar
- Forrest LM, McMillan DC, McArdle CS, Angerson WJ, Dagg K, Scott HR. A prospective longitudinal study of performance status, an inflammation-based score (GPS) and survival in patients with inoperable non-small-cell lung cancer. Br J Cancer. 2005;92:1834–6.View ArticlePubMedPubMed CentralGoogle Scholar
- McMillan DC. An inflammation-based prognostic score and its role in the nutrition-based management of patients with cancer. Proc Nutr Soc. 2008;67:257–62.View ArticlePubMedGoogle Scholar
- Wang JY, Tang R, Chiang JM. Value of carcinoembryonic antigen in the management of colorectal cancer. Dis Colon Rectum. 1994; 37:272–7.Google Scholar
- Nakagoe T, Sawai T, Tsuji T, et al. Preoperative serum level of CA19-9 predicts recurrence after curative surgery in node-negative colorectal cancer patients. Hepatogastroenterology. 2003;50(51):696-9.Google Scholar
- Nozoe T, Mori E, Takahashi I, Ezaki T. Preoperative elevation of serum C-reactive protein as an independent prognostic indicator of colorectal carcinoma. Surg Today. 2008;38:597–602.Google Scholar
- Lai CC, You JF, Yeh CY, Chen JS, Tang R, Wang JY, et al. Low preoperative serum albumin in colon cancer: a risk factor for poor outcome. Int J Colorectal Dis. 2011;26:473–81.Google Scholar
- Kawaguchi H, Ohno S, Miyazaki M, et al. CYFRA 21-1 determination in patients with esophageal squamous cell carcinoma: clinical utility for detection of recurrences. Cancer. 2000;89:1413–17.Google Scholar
- Takashi Kobayashi, Masanori Teruya, Tomokazu Kishiki, et al. Inflammation-based prognostic score, prior to neoadjuvant chemoradiotherapy, predicts postoperative outcome in patients with esophageal squamous cell carcinoma. Surgery. 2008;144(5):729–35. Google Scholar
- Kobayashi T, Teruya M, Kishiki T, et al. Inflammation-Based Prognostic Score and Number of Lymph Node Metastases Are Independent Prognostic Factors in Esophageal Squamous Cell Carcinoma. Dig Surg. 2010;27(3):232–7.Google Scholar
- Nakamura M, Iwahashi M, Nakamori M. A new prognostic score for the survival of patients with esophageal squamous cell carcinoma. Surg Today. 2014;44(5):875–83.Google Scholar
- Vashist YK, Loos J, Dedow J, Tachezy M, et al. Glasgow Prognostic Score is a predictor of perioperative and long-term outcome in patients with only surgically treated esophageal cancer. Ann Surg Oncol. 2011;18(4):1130–8.Google Scholar
- Crumley AB, McMillan DC, McKernan M, McDonald AC, Stuart RC. Evaluation of an inflammation-based prognostic score in patients with inoperable gastro-oesophageal cancer. Br J Cancer. 2006; 94:637–41.Google Scholar
- Forrest LM, McMillan DC, McArdle CS, Angerson WJ, Dunlop DJ. Comparison of an inflammation-based prognostic score (GPS) with performance status (ECOG) in patients receiving platinum-based chemotherapy for inoperable non-small-cell lung cancer. Br J Cancer. 2004;90(9):1704–6.Google Scholar
- McMillan DC, Scott HR, Watson WS, Preston T, Milroy R, McArdle CS. Longitudinal study of body cell mass depletion and the inflammatory response in cancer patients. Nutr Cancer. 1998; 31:101–5.Google Scholar
- Tanigawa N, Amaya H, Matsumura M, Shimomatsuya T. Correlation between expression of vascular endothelial growth factor and tumor vascularity, and patient outcome in human gastric carcinoma. J Clin Oncol. 1997;15:826–32.29. Google Scholar
- Fondevila C, Metges JP, Fuster J, Grau JJ, Palacin A, Castells A, et al. p53 and VEGF expression are independent predictors of tumour recurrence and survival following curative resection of gastric cancer. Br J Cancer. 2004;90:206–15.Google Scholar
- Roxburgh CS, Salmond JM, Horgan PG, Oien KA, McMillan DC. Comparison of the prognostic value of inflammation-based pathologic and biochemical criteria in patients undergoing potentially curative resection for colorectal cancer. Ann Surg. 2009;249:788–93.Google Scholar