Fig. 5

LINC02086 promotes EPHA2 expression via miR-6757-5p. A, the binding site between miR-6757-5p and EPHA2; B, luciferase reporter activity in MCF-7 cells transfected withmiR-mimic/miR-inhibitor and wild type or mutant LINC02086; C-D, EPHA2 mRNA and protein levels in MCF-7 cells transfected with miR-mimic or miR-inhibitor; E, EPHA2 mRNA levels in paired cancer specimens and adjacent-normal specimens; F, correlations between EPHA2 mRNA levels and miR-6757-5p mRNA levels; G-H, EPHA2 mRNA and protein levels in MCF-7 cells co-transfected with shRNA-1/shNC and miR-inhibitor/miR-NC; I, correlations between EPHA2 mRNA levels and LINC02086 levels. *P < 0.05, **P < 0.01, ***P < 0.001 vs.miR-NC, normal or shNC + miR-NC. ##P < 0.01, ###P < 0.001 vs. shRNA-1 + miR-NC