Fig. 3

LINC02086 interacts with and negatively correlates with miR-6757-5p. A cytoplasmic/nuclear fractionation of LINC02086 in MCF-7 and MDA-MB-231 cells; B, the binding site between LINC02086 and miR-6757-5p; C, LINC02086 levels in MCF-7 cells transfected with miR-inhibitor or miR-mimic; D, luciferase reporter activity in MCF-7 cells transfected with miR-mimic and wild type or mutant LINC02086. E, miR-6757-5p enrichment with LINC02086 detected by RNA pull down assay. F, LINC02086 levels in MCF-7 cells transfected with miR-inhibitor or miR-mimic; G, miR-6757-5p levels in 43 paired cancer specimens and adjacent-normal specimens; H, correlations of miR-6757-5p levels with LINC02086 levels in cancer specimens. *P < 0.05, **P < 0.01, ***P < 0.001 vs. miR-NC, control probe or normal