Table 1 Primer sequences and amplified fragments
From: Role of hypermethylated SLC5A8 in follicular thyroid cancer diagnosis and prognosis prediction
Primer | Forward primer | Reverse primer | Length |
|---|---|---|---|
SCL5A8 | 5′-ATCTACGGTGTCAACCAATCCC-3′ | 5′-GCGAGCCCACAAAACACTGAG-3′ | 134 bp |
SCL5A8 unmethylation | 5′TTGAATGTATTTTGAGGTG-3′ | 5′-TCAATTTTCCAAAATCCC-3′ | 100 bp |
SCL5A8 methylation | 5′-TCGAACGTATTTCGAGGC-3′ | 5′-ACAACGAATCGATTTTCCG-3′ | 108 bp |
β-actin | 5′-GTGGACATCCGCAAAGAC-3′ | 5′-AAAGGGTGTAACGCAACTAA-3′ | 302 bp |