Preliminary study on miRNA in prostate cancer

Table 1 miRNA real-time fluorescence quantification reaction conditions

Steps	Temperature	Time	Number of cycles
Predegeneration	95℃	30 s
Degeneration	95℃	5 s	40 cycles
Annealing/Extension	60℃	30 s	40 cycles

The melting curve analysis should be performed according to the recommended procedure of the fluorescence PCR instrument used

ISSN: 1477-7819