Fig. 5

Overexpression of RECQL4 abolished the effect of TRIM58 overexpression in CRC cells. SW620 cells were transduced with TRIM58 and/or RECQL4 expression vector. A Cell viability was measured using CCK-8 assay. B, C Cell cycle was measured through flow cytometry. D Protein levels of AKT, p-AKT, p21, and cyclin D1 were measured through Western blotting. *p < 0.05, ***p < 0.001 vs. oeNC. #p < 0.05, ###p < 0.001 vs. oeTRIM58