Fig. 4

TRIM58 interacted with RECQL4 and enhanced its ubiquitination in CRC cells. A The relative mRNA levels of RECQL4 in SW620 cells transduced with RECQL4 expression vector. B The protein levels of RECQL4 in SW620 cells transduced with RECQL4 expression vector and treated with MG132. C Co-IP assay shows that TRIM58 interacted with RECQL4 in SW620 cells. D Ubiquitination assay was performed to examine the ubiquitination of RECQL4 in LOVO cells transduced with TRIM58 shRNA and SW620 cells transduced with TRIM58 expression vector. E Immunohistochemistry staining was performed to examine the protein expression of TRIM58 and RECQL4 in CRC and paracancerous tissues (scale bar, 100 μm)