Fig. 3
From: Lung cancer-derived exosomal miR-132-3p contributed to interstitial lung disease development
MiR-132-3p in exosomes activated the normal human lung fibroblast. A MiR-132-3p had a high expression in the non-small-cell lung tumor-derived exosomal miRNAs. B The qRT-PCR tested the expression of miR-132-3p in exosomes from A549 cells and the corresponding cells. C In NHLF, the transfection efficiency of miR-132-3p mimic or inhibitor was evaluated. D MTT assays tested the cell viability in miR-132-3p mimic, miR-132-3p inhibitor, and miR-NC groups. E Clone formation assays tested the cell proliferation in all groups. F Flow cytometry analyzed the apoptosis rates in all groups. G Western blot assay was performed to analyze the expression levels of Bax, Bcl-2, and cleaved caspase-3 in NHLF transfected with miR-132-3p mimic, miR-132-3p inhibitor, or miR-NC. H-I. The mRNA expressions, including α-SMA, FN, TGF-β, COL1A1, COL3A1, CXCL12, and IL-1β/6/8, were tested by qRT-PCR analysis. Data were presented as mean ± SD of three independent experiments. *P < 0.05; **P < 0.01, ***P < 0.001