Fig. 2

The exosomes from A549 cells activated the normal human lung fibroblast. A The green fluorescence levels were detected for NHLF co-cultured with PKH67-labeled exosomes from A549 cells. B MTT assay tested the viability of NHLF treated with exosomes or exosomes + GW4869. C The proliferation abilities of NHLF, which were incubated with exosomes or exosomes + GW4869, were checked by clone formation assay. D The expression levels of proliferation-related proteins (Ki-67 and PCNA) were tested for control, exo and exo + GW4869 group. E Flow cytometry assay was performed to analyze the apoptosis rates in all groups. F Western blot analysis of the pro-/anti-apoptosis proteins (Bax and Caspase-3 and Bcl-2) was performed. G–H α-SMA, FN, TGF-β, COL1A1, COL3A1, CXCL12, IL-1β, IL-6, and IL-8 mRNA expressions were tested by qRT-PCR analysis. Data were presented as mean ± SD of three independent experiments. ***P < 0.001; **P < 0.01