Fig. 7

RNF145 is up-regulated in hypoxic HCC cells and its inhibition attenuates proliferation of HCC cells. A The mRNA level of RNF145 in normoxic or hypoxic Hep3B and Huh7 cells by use of qRT-PCR. B, C The protein level of RNF145 in normoxic or hypoxic HCC cells utilizing western blot. D Verification of the transfection effect of si-RNF145 in HCC cells at the mRNA level. E Representative photographs of HCC cells with si-RNF145 transfection. Bar, 50 μm. F The cell viability of RNF145-knockout HCC cells utilizing CCK-8. In comparison to si-NC group, **p<0.01; ***p<0.001; ****p<0.0001