Fig. 2

Two hypoxia subtypes characterized by distinct signaling pathways, immune cell infiltration, PD-L1 expression, and genetic mutation across TCGA-LIHC samples. A GSEA of the major KEGG pathways with different enrichment in two hypoxia subtypes. B Landscape of the relative infiltration of immune cells across TCGA-LIHC tissues. C, D Comparison of immune cell infiltration between hypoxia subtypes. E Difference in PD-L1 expression between cluster1 and cluster2. F Distribution of TMB score across TCGA-LIHC samples. G Difference in TMB score between subtypes. H, I waterfall plots of the somatic landscape in two hypoxia subtypes cluster1 and cluster2. Genes are ranked according to mutational frequency. Side bar plot displays the percentage of mutated samples. J, K Mutually exclusive and co-occurring mutated genes in cluster1 and cluster2. Green denotes co-occurrence and brown denotes exclusivity. L Comparison of mutated genes between cluster1 and cluster2. M Differentially mutated genes between subtypes. Bar indicates 95% confidence interval of odd ratio (OR). The right table displays the number of samples in two subtypes with the mutation in the indicated gene. *P<0.05; **p<0.01; ***p<0.001; ****p<0.0001