Fig. 4

Hyperglycemia might promote PFKFB3 expression by miR-26 downregulation in breast cancer. A TargetScan database, B OncomiR database, and C miRcode database were utilized to predict that miR-26 was a reliable upstream microRNA of PFKFB3. D Protein levels of PFKFB3, E-cadherin, N-cadherin, and Vimentin in BT474-25 mM or MCF-7-25 mM cells after transfected with miR-26-mimic, miR-26-inhibitor, or corresponding-NC were detected by western blot. β-actin was detected as control. *, P < 0.05; **, P < 0.01