Fig. 2

PFKFB3 might activate epithelial–mesenchymal transition and RAS/MAPK pathways of breast cancer in a hyperglycemic environment. A GSEA (Gene Set Enrichment Analysis) was performed to investigate the downstream signaling pathways. Pathway lists of PFKFB3 screened out by GSEA were shown on the left, the box plots of EMT (upper) and RAS/MAPK pathway (lower) were shown on the right. B Protein levels of PFKFB3, E-cadherin, N-cadherin, Vimentin, p-ERK1/2, and t-ERK1/2 in BT474-25 mM or MCF-7-25 mM cells after transfected with siPFKFB3-1, siPFKFB3-2 or siNC were detected using western blot. β-actin was detected as control. *, P < 0.05; **, P < 0.01