Fig. 5

Correlation analysis of CD73 expression and immune cell infiltration in ICC. A Immune cell infiltration levels between high and low CD73 mRNA groups in the FU-iCCA cohort estimated by CIBERSORT. B Images of positive CD8 and Foxp3 staining and negative controls (400 × , scale bar, 50 μm). C Representative images of positive CD68 and CD163 staining (400 × , scale bar, 50 μm). D Violin plots depicting the correlation between CD73 expression and CD8 + TILs, Foxp3 + Tregs, CD68 + TAMs, and CD163 + TAMs. Violin plots showing the correlation between CD73 expression and Foxp3 + /CD8 + TIL ratio (E) as well as CD163 + /CD68 + TAM ratio (F). TIL, tumor-infiltrating lymphocytes; Tregs, regulatory T cells; TAMs, tumor-associated macrophages. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001