Fig. 4

Biological functions of CD73 in ICC. A Efficiency of CD73 knockdown validated by RT-PCR (left) and WB (right). Evaluation of the effect of CD73 knockdown on proliferation by CCK-8 (B) and colony formation assays (C). Evaluations of the influence of CD73 knockdown on the cell cycle (D) and apoptosis (E) by flow cytometry. Evaluations of the influence of CD73 knockdown on migration and invasion of ICC cells by wound healing assays (F) and Transwell assays (G). H Gene set enrichment analysis (GSEA) of differential hallmark gene sets between CD73 high/low groups in the FU-iCCA cohort. Expression of EMT-related markers in ICC cells with CD73 knockdown (I) or overexpression (J) by RT-PCR assays and WB assays (K). EMT, epithelial-mesenchymal transition; WB, western blot. *P < 0.05, **P < 0.01, ***P < 0.001