Fig. 8

ANGPT2 blocks the suppression of PD-L1 expression by HOXD9 in NSCLC cells. NCI-H292 cells were co-transfected with siHOXD9 and exANGPT2 or empty vector. A After 48 h of transfection, total PD-L1 protein expression in NCI-H292 cells was evaluated by western blot assay. B After 48 h of transfection, membrane PD-L1 protein expression in NCI-H292 cells was evaluated by flow cytometry analysis. MFI, relative mean fluorescence intensity. * p < 0.05. Data in (B) are presented as mean values ± SD. The blots were cropped and the original uncropped images of blots were shown in supplementary materials