Fig. 7

HOXD9 has the ability to stimulate PD-L1 expression in NSCLC cells. NCI-H661 cells were transfected with exHOXD9 or empty vector, while NCI-H292 cells were transfected with siHOXD9^#1, siHOXD9^#2 or their negative control siNC. A After 48 h of transfection, total PD-L1 protein expression in two NSCLC cells (NCI-H661 and NCI-H292) was evaluated by western blot assay. B After 48 h of transfection, soluble PD-L1 protein expression in two NSCLC cells was evaluated by ELISA assay. C After 48 h of transfection, membrane PD-L1 protein expression in NCI-H292 cells was evaluated by flow cytometry analysis. MFI, relative mean fluorescence intensity. ** p < 0.01; *** p < 0.001. Data in (B) and (C) are presented as mean values ± SD. The blots were cropped and the original uncropped images of blots were shown in supplementary materials