Fig. 6

ANGPT2 is involved in the anti-migratory and anti-invasive effects of HOXD9 silencing in NSCLC cells. NCI-H292 cells were co-transfected with siHOXD9 and exANGPT2 or empty vector. NCI-H661 cells were co-transfected with exHOXD9 and siANGPT2 or siNC. (A and B) After 48 h of transfection, the migratory ability and invasiveness of NCI-H292 (A) and NCI-H661 (B) cells were evaluated by scratch-wound healing assay (Scale bar = 200 μm) and transwell assay (Scale bar = 100 μm). ** p < 0.01; *** p < 0.001. Data in (A) and (B) are presented as mean values ± SD