Fig. 4

ANGPT2 is a direct transcriptional target of HOXD9. A Correlation analysis of HOXD9 with ANGPT2 in NSCLC using the UALCAN and TIMER databases. B NCI-H661 cells were transfected with exHOXD9 or empty vector, while NCI-H292 cells were transfected with siHOXD9^#1, siHOXD9^#2 or their negative control siNC. After 48 h of transfection, ANGPT2 mRNA expression in two NSCLC cells (NCI-H661 and NCI-H292) was evaluated by real time-PCR assay. C After 48 h of transfection, ANGPT2 protein expression in two NSCLC cells was evaluated by western blot assay. D HEK293T cells were co-transfected with a pGL3-basic vector containing the promoter sequence of homo ANGPT2, pRL-TK, and exHOXD9 or empty vector. After 24 h of transfection, ANGPT2 luciferase activity was evaluated by dual-luciferase reporter assay. * p < 0.05; *** p < 0.001. Data in (B) and (D) are presented as mean values ± SD. The blots were cropped and the original uncropped images of blots were shown in supplementary materials