Fig. 3

HOXD9 promotes migration and invasion of NSCLC cells. NCI-H661 cells were transfected with exHOXD9 or empty vector, while NCI-H292 cells were transfected with siHOXD9 or siNC. A After 48 h of transfection, the migratory ability of two NSCLC cells (NCI-H661 and NCI-H292) was evaluated by scratch-wound healing assay. The wound healing was monitored at 0 h and 24 h post-scratching, and the percentage of wound closure was measured. Scale bar = 200 μm. B After 48 h of transfection, the invasiveness of two NSCLC cells was assessed by transwell assay. The invaded cells were counted in five random fields. Scale bar = 100 μm. ** p < 0.01; *** p < 0.001. Data in (A) and (B) are presented as mean values ± SD