Fig. 1

HOXD9 is an upregulated gene that predicts worse overall survival in patients with NSCLC. A HOXD9 mRNA levels in NSCLC and normal lung tissues obtained from GSE118370 were detected (left). HOXD9 mRNA levels in 30 paired NSCLC and para-NSCLC tissues from our independent hospital cohorts were detected via real time-PCR (right). B HOXD9 protein levels in 10 paired NSCLC and para-NSCLC tissues from our independent hospital cohorts were detected via western blot. P, para-NSCLC tissue; C, NSCLC tissue. C The effect of HOXD9 on the overall survival in NSCLC patients via Kaplan–Meier Plotter. Patients with NSCLC expressing high HOXD9 levels are labeled in blue, whereas those with tumors with low HOXD9 levels are shown in black. ** p < 0.01; *** p < 0.001. Data in (A) are presented as mean values ± SD. The blots were cropped and the original uncropped images of blots were shown in supplementary materials