Table 3 Univariate and multivariate Cox regression of clinicopathological features and infiltrating immune cells for overall survival

Univariate analysis

 sTILs

0.000

0.553 (0.398–0.769)

0.002

0.499 (0.321–0.776)

0.162

0.704 (0.431–1.151)

 iTILs

0.497

1.413 (0.521–3.831)

0.949

1.047 (0.256–4.281)

0.201

2.533 (0.609–10.546)

 CD3*

0.001

0.478 (0.307–0.745)

0.012

0.518 (0.309–0.868)

0.014

0.318 (0.127–0.796)

 CD20*

0.007

0.613 (0.428–0.877)

0.029

0.600 (0.379–0.950)

0.044

0.573 (0.333–0.985)

 Age (< 60 years)

0.013

1.731 (1.124–2.666)

0.156

1.410 (0.877–2.268)

0.232

21.827 (0.139–3436.270)

 Gender (male/female)

0.013

1.562 (1.100–2.216)

0.022

1.738 (1.082–2.790)

0.307

1.311 (0.779–2.207)

 Tumor size (5 cm)

0.834

1.036 (0.746–1.437)

0.526

0.864 (0.550–1.357)

0.320

1.282 (0.786–2.089)

Tumor site

 Rectum

Refer

Refer

Refer

 Left colon

0.908

1.025 (0.676–1.553)

0.677

0.891 (0.516–1.537)

0.539

1.229 (0.636–2.376)

 Right colon

0.501

0.869 (0.578–1.307)

0.054

0.587 (0.341–1.010)

0.128

1.636 (0.867–3.084)

 Pathological T stage

0.000

2.596 (1.565–4.307)

0.001

3.375 (1.625–7.008)

0.087

1.851 (0.915–3.747)

 Lymph node metastasis

0.000

2.793 (2.002–3.895)

0.000

2.469 (1.581–3.856)

0.000

3.552 (2.141–5.894)

 TNM stage

0.000

3.216 (2.279–4.537)

0.000

2.797 (1.765–4.434)

0.000

4.219 (2.479–7.128)

 Tumor differentiation

0.000

1.879 (1.326–2.662)

0.003

2.013 (1.260–3.216)

0.054

1.668 (0.991–2.809)

 Lymphangio

0.000

2.038 (1.467–2.832)

0.000

2.829 (1.811–4.419)

0.275

1.321 (0.801–2.180)

 Nervous invasion

0.143

1.492 (0.873–2.552)

0.327

1.417 (0.706–2.844)

0.500

1.727 (0.741–4.024)

 Tumor deposit

0.000

3.979 (2.667–5.936)

0.000

3.967 (2.353–6.688)

0.000

4.138 (2.205–7.769)

 Colonic perforation

0.813

0.887 (0.328–2.397)

0.767

1.190 (0.375–3.670)

0.508

0.506 (0.070–3.657)

 Tumor budding (≤ 5 cells)

0.001

1.877 (1.297–2.715)

0.000

2.500 (1.492–4.188)

0.421

1.247 (0.729–2.134)

 Schistosomiasis

0.049

1.390 (1.001–1.929)

—

—

—

—

 Ulceration

0.617

0.919 (0.659–1.281)

0.743

1.077 (0.690–1.681)

0.212

0.725 (0.437–1.201)

 Histological type

0.705

1.096 (0.683–1.757)

0.280

1.403 (0.759–2.594)

0.467

0.760 (0.362–1.594)

Multivariate analysis

 Gender

0.001

1.886 (1.295–2.746)

0.021

1.812 (1.093–3.004)

—

 TNM stage

0.000

2.099 (1.403–3.139)

—

—

0.000

3.703 (2.149–6.381)

 Tumor budding (≤ 5 cells)

—

—

0.050

1.647 (0.988–2.745)

—

—

 Tumor differentiation

0.039

1.499 (1.021–2.203)

0.021

1.826 (1.095–3.044)

—

 CD3*

0.003

0.481 (0.297–0.781)

0.026

0.525 (0.297–0.927)

0.045

0.404 (0.160–1.021)

 sTILs

0.031

0.660 (0.452–0.963)

0.034

0.570 (0.339–0.959)

—

—

 Lymphangio

0.012

1.580 (1.106–2.256)

0.006

1.994 (1.223–3.252)

—

—

 Tumor deposit

0.000

2.818 (1.743–4.555)

0.000

3.837 (2.053–7.170)

—

—