Fig. 5
YTHDF1 enhanced the stability of METTL4-2 via m6A modification. A The expression of m6A “reader” (such as YTHDC1, YTHDF1, and IGF2BP) was detected by the TCGA database. B qRT-PCR detected YTHDF1 expression in CSCC tissues and normal tissues (n=8). C qRT-PCR detected YTHDF1 expression in CSCC metastatic tissues and non-metastatic tissues (n=8). D RIP assay was performed in CSCC cells using an anti-YTHDF1 antibody, followed by the qRT-PCR analysis of METTL4-2 enrichment (n=3). E The transfection efficiency of YTHDF1 silencing by qRT-PCR (n=3). F The effect of YTHDF1 silencing on METTL4-2 expression was detected by qRT-PCR (n=3). G RNA stability analysis of METTL4-2 in treated C33A cells (n=3). *p < 0.05; **p < 0.01; ***p < 0.001