Fig. 4
SLERT increases BDNF m6A level via METTL3/IGF2BP1 axis. A–D Detection of BDNF m6A levels in KLE and AN3CA cells with SLERT knockdown or overexpression. E, F qRT-PCR analysis of BDNF mRNA expression in SLERT-overexpressed EC cells transfected with METTL3 siRNA. G–I RNA pull-down and RIP assays testing the interaction between METTL3 and SLERT in EC cells. J, K qRT-PCR analysis of BDNF mRNA expression in SLERT-overexpressed EC cells transfected with IGF2BP1 siRNA. L–O RIP assay testing the enrichment of METTL3 or IGF2BP1 on BDNF mRNA after SLERT silencing. *P<0.05, **P<0.01, ***P<0.001