Fig. 3

SLERT increases BDNF mRNA expression. A–D TCGA-EC database showing the correlations between SLERT and E-cad, VIM, MMP9, and BDNF. E The cartoon showing the construction of pGL3-basic vector containing BDNF promoter. F–H Luciferase reporter assay in HEK293T, KLE, and AN3CA cells with SLERT knockdown. I, J qRT-PCR analysis of BDNF mRNA level in SLERT-silenced KLE and AN3CA cells after treatment with Actinomycin D. K, L Transwell testing the invasion of SLERT-silenced KLE and AN3CA cells transfected with SLERT or BDNF expression plasmid. *P<0.05, **P<0.01