Fig. 1

Flow cytometric analysis of TILs subsets. A representative example of the flow cytometric gating strategy used for the immune checkpoint receptor analysis of NK cell subsets and cytotoxic T cells is shown. Tumor infiltrated lymphocytes were identified by their size and granularity in the FSC/SSC dot plot, and NK cells were gated on the SSC/CD3- and then gated further as CD56^bright or CD56^dim NK cells. Cytotoxic T cells were identified by gating on CD3⁺/CD8⁺ quadrant. PD-1, TIGIT, CTLA-4, LAG-3, and Tim-3 expressions were evaluated on CD8⁺ T cells and cytotoxic (CD16⁺CD56^dim) and cytokine secreting (CD16^-CD56^bright) NK cells. PD-1, TIGIT, CTLA-4, LAG-3, and Tim-3 expressions are given as percentage of the designated population. Data were analyzed using the FlowJo^TM10.2 software