Fig. 6

Effects of miR-1827 and SLC1A5 on NSCLC cell proliferation, apoptosis, migration, and invasion. A The transfection efficiency of pcDNA SLC1A5 overexpression vector was confirmed by measuring SLC1A5 protein expression by WB analysis. B–K) A549 and NCI-H1299 cells were transfected with miR-NC, miR-1827, miR-1827 + pcDNA, or miR-1827 + SLC1A5. B The protein expression of SLC1A5 was measured by WB analysis. CCK8 assay (C–D), colony formation assay (E), and EdU staining (F) were performed to detect cell proliferation. Cell apoptosis, migration, and invasion were determined by flow cytometry (G), wound healing assay (H), and transwell assay (I). J–K The protein levels of ki67, Bax, and E-cadherin were measured by WB analysis. *P < 0.05, **P < 0.01, ***P < 0.001