Fig. 6

Inhibition of LINC00922 suppresses GC progression via targeting miR-204-5p /HMGA2 axis in vitro. (A) The transfection efficiency of miR-204-5p inhibitor was certified by qRT-PCR assay. *P < 0.05, **P < 0.01, ***P < 0.001 vs. NC inhibitor group. B The transfection efficiency of sh-HMGA2 was certified by qRT-PCR assay. *P < 0.05, **P < 0.01, ***P < 0.001 vs. sh-NC group. C, D qRT-PCR and western blot assays were used to assess HMGA2 expression in MKN-45 and HGC-27 cells with miR-204-5p inhibitor and/or sh-HMGA2 transfection. MKN-45/sh-LINC00922 and HGC-27/sh-LINC00922 cells were co-transfected with miR-204-5p inhibitor and/or sh-HMGA2. E CCK-8 assay was performed to investigate the proliferation. F Transwell assay was used to investigate the migration and invasion. G Flow cytometry assay was used to assess cell apoptosis. **P < 0.01, ***P < 0.001 vs. NC inhibitor + sh-NC group, #P < 0.05, ## P < 0.01 vs. miR-204-5p inhibitor + sh-NC group