Fig. 5

Inhibition of MSTO2P suppresses CRC progression through upregulating CDKN1A expression. si-MSTO2P and si-CDKN1A were co-transfected into HT-29 cells. A CCK-8 assay showed that cell viability was inhibited by si-MSTO2P, which was reversed by si-CDKN1A. B, C Colony formation assay clarified that si-MSTO2P decreased the number of colonies in HT-29, while si-CDKN1A partially recovered clone formation. D, E Transwell assay showed cell invasion in HT-29 cells. Bar values= 100 μm. F, G The cell cycle and (H–I) cell apoptosis were detected by flow cytometry assay