Fig. 6

The upregulation of BECN1 reversed miR-326-induced the effect on lung adenocarcinoma cells. A The expression level of miR-326 was determined by RT-qPCR in A549 and HCC827 cells transfected with miR-con or miR-326. B The protein expression of BECN1 was quantified by western blot assay in A549 and HCC827 cells transfected with pcDNA or BECN1. C–N A549 and HCC827 cells were transfected with miR-con, miR-326, miR-326+pcDNA, or miR-326+BECN1. C–D Western blot assay was conducted in transfected A549 and HCC827 cells to assess BECN1 level. E–H The migration and invasion were measured by wound healing and transwell assays, respectively. I, J Cell proliferation was analyzed by colony-forming assay. K, L The tube formation was determined by matrigel angiogenesis assay. M, N The apoptosis was assessed by flow cytometry assay. *P < 0.05