Fig. 2

Circ_0026416 downregulation inhibited CRC development in vitro and in vivo. A The expression of circ_0026416 in HCT-8 and SW480 cells after si-circ_0026416 transfection was checked by qPCR. B, C Cell proliferation was checked by CCK-8 assay. D Cell proliferation was also checked by colony formation assay. E, F Cell migration and cell invasion were examined using Transwell assay. G The ability of angiogenesis was checked by tube formation assay. H Cell apoptosis was examined using flow cytometry assay. I, J The protein levels of E-Cadherin, vimentin, and N-Cadherin were determined by western blot. K–M Animal study was performed to determine the role of circ_0026416 knockdown in vivo. N The expression of circ_0026416 in tumor tissues from animal model was checked by qPCR. *P < 0.05