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Fig. 4 | World Journal of Surgical Oncology

Fig. 4

From: Circ_0029803 serves as the sponge of miR-216b-5p to promote the progression of colorectal cancer by regulating SKIL expression

Fig. 4

Circ_0029803 knockdown suppressed the progression of CRC cells by targeting miR-216b-5p. A, B MiR-216b-5p expression in HCT-116 and SW480 cells transfected with si-NC, si-circ_0029803, si-circ_0029803 + anti-miR-NC, or si-circ_0029803 + anti-miR-216b-5p was examined by qRT-PCR. C, D The proliferation of transfected HCT-116 and SW480 cells was measured using colony formation assay. E, F The apoptosis rate of transfected HCT-116 and SW480 cells was detected by Flow cytometry. G, H The migration and invasion of transfected HCT-116 and SW480 cells were detected by Transwell assay. I, J The levels of E-cadherin, N-cadherin, and Vimentin were checked by western blot. KN Glucose consumption and lactate production of transfected HCT-116 and SW480 cells were measured using a glucose and lactate assay kit. O, P The protein level of HK in transfected HCT-116 and SW480 cells was measured by western blot. *P<0.05

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