Fig. 6

MiR-183-5p-mediated inhibition of TMED5 impeded cervical cancer development and angiogenesis in vitro. A, B TMED5 mRNA and protein levels by qRT-PCR and western blot assays, respectively, in SiHa and HeLa cells transfected with pcDNA-TMED5 or negative control pcDNA. SiHa and HeLa cells were transfected with miR-183-5p mimic, miR-NC mimic, miR-183-5p mimic + pcDNA, or miR-183-5p mimic + pcDNA-TMED5, followed by the assessment of cell proliferation by CCK-8 (C) and Edu (D) assays, cell apoptosis by flow cytometry (E), and Bax and Bcl-2 levels by western blot (F). G Tube formation assay performed with human HUVECs pre-treated with the conditional medium of SiHa and HeLa cells transfected with miR-183-5p mimic, miR-NC mimic, miR-183-5p mimic + pcDNA, or miR-183-5p mimic + pcDNA-TMED5. H Western blot showing VEGFA and FGF2 levels in SiHa and HeLa cells transfected with miR-183-5p mimic, miR-NC mimic, miR-183-5p mimic + pcDNA, or miR-183-5p mimic + pcDNA-TMED5. *P < 0.05