Fig. 4
From: MiR-186-3p attenuates tumorigenesis of cervical cancer by targeting IGF1
IGF1 and miR-186-3p have opposite effects in HeLa and SiHa cells. A qRT-PCR detection of the relative expression levels of miR-186-3p and IGF1 in HeLa and SiHa cells transfected with Si-IGF1, inhibitor and inhibitor + Si-IGF1. B MTT assay determination of viability of HeLa and SiHa cells transfected with Si-IGF1, inhibitor and inhibitor + small interfering-(Si) IGF1. C Cell proliferation of HeLa and SiHa by BrdU assay was determined in HeLa and SiHa cells transfected with Si-IGF1, inhibitor and inhibitor + Si-IGF1. D Transwell assay measurement of migration of HeLa and SiHa cells transfected with Si-IGF1, inhibitor and inhibitor + Si-IGF1. E Detection of caspase-3 activity of HeLa and SiHa cells transfected with Si-IGF1, inhibitor and inhibitor + Si-IGF1. F Western blotting detected the expression of cleaved caspase-3 and total caspase-3 in HeLa and SiHa cells transfected with Si-IGF1, inhibitor and inhibitor + Si-IGF1. * P < 0.05; ** P < 0.001 compared with CON group using ANOVA. # P < 0.05; ## P < 0.001 compared with inhibitor + Si-IGF1 group using ANOVA. CON, blank control. NC, negative control; Si-IGF1, SiRNA-IGF1; inhibitor, miR-186-3p inhibitor