Fig. 6

MiR-330-5p restrains PCa cell growth and metastasis via targeting NRBP1. A qRT-PCR analysis of miR-330-5p expression in PC3 and DU145 cells transfected with miR-330-5p mimic or mimic NC. B–J PC3 and DU145 cells were transfected with mimic NC, miR-330-5p mimic, miR-330-5p mimic + pcDNA, or miR-330-5p mimic + pcDNA-NRBP1. B Western blot analysis of NRBP1 expression in cells. C–E Cell proliferation were determined by CCK-8 assay (C), colony formation assay (D), and EDU incorporation assay (E). F, G Flow cytometry for cell cycle and apoptosis. H Transwell assay for cell invasion. I Wound healing assay for cell migration. J Western blot analysis of the expression of E-cadherin and Vimentin. *P < 0.05