Fig. 2

Knockdown of circLRP6 suppresses PCa cell growth and metastasis. A Analysis of the interference efficiency of si-circLRP6 (si-circLRP6#1, si-circLRP6#2, or si-circLRP6#3) in PC3 and DU145 cells using qRT-PCR. B–I PC3 and DU145 cells were transfected with si-circLRP6#1, si-circLRP6#2, or si-NC. B–D Cell proliferation was determined by CCK-8 assay (B), colony formation assay (C), and EDU incorporation assay (D). E Flow cytometry for cell cycle. F Transwell assay for cell invasion. G Wound healing assay for cell migration. H Flow cytometry for cell apoptosis. I Western blot analysis of the expression of E-cadherin and Vimentin. *P < 0.05