Fig. 4

CircASXL1 modulated CRC development by associating with miR-1205. A The high silencing efficiency of miR-1205 inhibitor was determined by qRT-PCR. B MiR-1205 inhibitor attenuated circASXL1 knockdown-mediated effects on cell colony-forming ability. C, D The effects of circASXL1 knockdown on cell cycle process and cell apoptosis were impaired by miR-1205 repression. E, F The impacts of circASXL1 silencing on the protein expression of Bcl-2 and Bax were reversed after miR-1205 repression. G–I The inhibitory effects of circASXL1 absence on the migration and invasion of SW480 and SW620 cells were restrained by miR-1205 inhibitor. Significant differences in (A) were compared with two-tailed Student’s t tests and in (B–I) with ANOVA.*P < 0.05