Fig. 3

miR-335 inhibited the proliferation of ovarian cancer cell lines through BCL2L2. A pcDNA3.1 BCL2L2 was transfected into ovarian cancer cell lines to accomplish BCL2L2 overexpression. B–E pcDNA3.1 BCL2L2 and miR-335/NC were co-transfected into SKOV3 and OVCAR3 cell lines. The experimental results of MTT and BrdU displayed that the cell proliferation rate was obviously decreased after transfection with the mimic of miR-335, while the overexpression of BCL2L2 turned over the apparent inhibition of cell proliferation by miR-335. F, G Overexpression of miR-335 apparently advanced DNA fragmentation in SKOV3 and OVCAR3 cells, while BCL2L2 transfection weakened the influence of miR-335 on DNA fragmentation in SKOV3 and OVCAR3 cells. *P < 0.05, **P < 0.01