Fig. 3

Circ_0055625 knockdown inhibited tumor progression and enhanced radiosensitivity in colon cancer. a The knockdown efficiency of si-circ_0055625#1, si-circ_0055625#2, or si-circ_0055625#3 was detected by qRT-PCR in SW480 and SW620 cells. b CCK-8 assay was performed to determine the effect of circ_0055625 silencing on the proliferation of SW480 and SW620 cells. c The impacts of circ_0055625 absence on the survival fraction of SW480 and SW620 cells after treatment of radiation (0, 2, 4, and 6 Gy) were investigated by colony formation assay. d The effects of circ_0055625 silencing on cell colony-forming ability with or without radiation treatment were investigated by colony formation assay in SW480 and SW620 cells. e Flow cytometry analysis was employed to elucidate the impacts of circ_0055625 depletion on cell apoptosis with or without radiation treatment in SW480 and SW620 cells. f, g Wound-healing and transwell migration assays were carried out to unveil the impacts of circ_0055625 repression on cell migration under radiation presence or absence in SW480 and SW620 cells. h Transwell invasion assay were carried out to unveil the impacts of circ_0055625 repression on cell invasion under radiation presence or absence in SW480 and SW620 cells. *P<0.05, **P<0.01, and ***P<0.001