Fig. 3

Overexpression of DSCR1-4 showed tumor suppressive function in CRC cells in vitro. a Western blot showed that overexpression of DSCR1-4 increased p21, p16, and hyperphosphorylation of NFAT1 and NFAT2, while decreased cyclin D1, CDK2, and CDK4. b CCK8 assays showed that overexpression of DSCR1-4 inhibited cell proliferation of CRC cells. Data are mean ± SEM of 5 replicates. c Overexpression of DSCR1-4 inhibited cell colony formation of CRC cells. Data are mean ± SEM of 3 replicates. d Overexpression of DSCR1-4 induced senescence of CRC cells by SA-β-gal staining. Two-tailed Student t test, *p < 0.05, **p < 0.01, ***p < 0.001