Fig. 4
Microscopic slide image showing Prussian blue staining in a 76-year-old male with hepatocellular carcinoma. a A low-power field image. We randomly selected five individual fields (blue boxes) of peritumoral liver parenchyma or outer liver parenchyma for each tumor. b, c High-power multispectral images of the peritumoral liver parenchyma (× 200 magnification). Using a multispectral imaging system, we could extract only Prussian blue signals