Fig. 2

ALKBH2 knockdown inhibits CRC cell proliferation. a qRT-PCR was performed to assess the relative ALKBH2 mRNA expression in Caco-2 and LOVO cells transfected with sh-ALKBH2 or sh-NC, **P < 0.01 vs. sh-NC. ALKBH2 mRNA expression is significantly reduced in cells transfected with sh-ALKBH2 compared to sh-NC group. b Western blotting was performed to quantify the relative ALKBH2 protein expression in Caco-2 and LOVO cells transfected with sh-ALKBH2 or sh-NC, **P < 0.01 vs. sh-NC. ALKBH2 protein expression is significantly reduced in cells transfected with sh-ALKBH2 compared to sh-NC group C. CCK-8 assay was performed to assess cell viability in Caco-2 and LOVO cells transfected with sh-ALKBH2 or sh-NC, **P < 0.01 vs. sh-NC. Knockdown of ALKBH2 significantly inhibits cell viability in both Caco-2 and LOVO cell lines