Fig. 2

Hepatic stellate cells (HSCs) increased HIF-1α expression in HCT116 colorectal cancer cells. HSCs promoted cancer cell migration. HCT116 colorectal cancer cells were co-cultured with HSC conditioned medium (HSC-CM). a PCR analysis for HIF-1α mRNA expression in cancer cells and b western blot analysis for HIF-1α expression in cancer cells (n = 4). c Scratch assays were performed and cells were examined after 24 h (n = 4). *P < 0.01 compared with controls